山茱萸生、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞影响的研究  

Effects of Dichloromethane Extraction Sites from Raw and Processed Fructus Corni on Ageing Human Embryonic Lung Cells Induced by D-galactose

作　　者：赵凤鸣[1] 赵青春[1] 黄艳[1] 杨柳[1] 武晓群[1] 周韬[1] 杜伟锋[2] 方泰惠[1] 王明艳[1] 

机构地区：[1]南京中医药大学,江苏南京210029 [2]浙江中医药大学,浙江杭州311401

出　　处：《辽宁中医杂志》2011年第12期2440-2443,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家"十五"科技攻关计划(2001BA701A11);国家自然科学基金(30772851)

摘　　要：目的:研究山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞影响。方法:以人胚肺细胞为材料,通过制备D-半乳糖细胞衰老模型,以MTT、SOD、NO为指标,观察D-半乳糖对人胚肺细胞的损伤以及山茱萸生品、制品二氯甲烷萃取部位的保护作用,并通过Rt-PCR检测,进一步探讨山茱萸生品、制品二氯甲烷萃取部位对衰老通路的影响。结果:山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞增殖有一定的影响;对D-半乳糖致衰人胚肺细胞抗氧化系统表现出保护作用;对衰老相关通路有一定的调控作用。结论:山茱萸生品、制品二氯甲烷萃取部位对D-半乳糖致衰人胚肺细胞有一定的保护作用。Objective：To investigate effects of dichloromethane extraction sites from raw and processed Fructus Corni on ageing human embryonic lung cells induced by D-galactose.Methods：Taking human embryonic lung cells as materials and MTT、SOD、NO as indicators,the impairment of human embryonic lung cells induced by D-galactose and protective function of dichloromethane extraction sites from raw and processed Fructus Corni were observed by making the cellular ageing model of D-galactose,and effects of dichloromethane extraction sites from raw and processed Fructus Corni on ageing pathways were further discussed by Rt-PCR test.Results：Dichloromethane extraction sites from raw and processed Fructus Corni exerted certain effect on proliferation of ageing human embryonic lung cells induced by D-galactose,showing protective effect on their antioxidant systems and having regulated effect on their pathways related to ageing.Conclusion：Dichloromethane extraction sites from raw and processed Fructus Corni have protective effects on ageing human embryonic lung cells induced by D-galactose.

关 键 词：山茱萸生品 山茱萸制品 二氯甲烷萃取部位 D-半乳糖 人胚肺细胞 

分 类 号：R284.2[医药卫生—中药学]