蛋白质氯胺T双相碘标法的建立及其应用  

Establishment of Methods for Two phase Iodination of Chromine T and Applying in Cytokine Receptor Studies

作　　者：从玉文[1] 陈家佩[1] 邵源 

机构地区：[1]北京放射医学研究所,北京100850

出　　处：《生物化学与生物物理进展》1999年第6期593-596,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金

摘　　要：常规的蛋白质碘标方法易引起被标细胞因子的失活， 是受体配基竞争结合实验失败的原因之一． 试用氯胺Ｔ 双相碘标法标记ｒｈＧＣＳＦ和ｒｈＥＰＯ， 并应用受体配基竞争结合分析法测定ＮＦＳ６０ 细胞ＧＣＳＦ受体及ＢＥＴ２细胞ＥＰＯ受体的特性． 结果显示所获１２５ＩＥＰＯ和１２５ＩＧＣＳＦ放射比活度均较高； 发现ＢＥＴ２ 细胞有高、低两种亲和力的ＥＰＯ受体， ＮＦＳ６０ 细胞只有一种高亲和力的ＧＣＳＦ 受体， 所获结果与文献资料相一致． 说明氯胺Ｔ双相碘标法是细胞因子同位素碘标记的理想方法之一．The routine iodination methods are easy to lead to biological inactivation of cytokines, which is one of reasons for failure of ligand receptor binding assay. By using two phase iodination of chromine T, rhG CSF and rhEPO were iodinated with Na 125 I and the characteristic of EPO receptor in BET 2 cell and G CSF receptor in NFS 60 cell were observed by receptor ligand binding assay. It was showed that the radioactivity of 125 I G CSF and 125 I EPO were much higher than that obtained by other methods and it was found that there are high and low affinity EPO receptors in BET 2 cells and one high affinity G CSF receptor in NFS 60 cells. These results are in accord with literature data. It suggests that the two phase iodination of chromine T is an ideal method to iodinate cytokines.

关 键 词：细胞因子 同位素碘标记 受体特性 蛋白质 双相法 

分 类 号：Q5－33[生物学—生物化学]