应用SMARTer cDNA合成技术扩增落地生根叶片的总RNA  

Total RNA Amplification from Leaves of Kalanchoe daigremontiana Using SMARTer cDNA Synthesis Technology

作　　者：钟天秀[1] 曾会明[1] 

机构地区：[1]北京林业大学草坪研究所,北京100083

出　　处：《草地学报》2011年第6期1051-1054,共4页Acta Agrestia Sinica

基　　金："十一五"国家863计划项目(2009AA10Z109)资助

摘　　要：以落地生根(Kalanchoe daigremontiana)未长芽和长芽的叶片为材料,提取落地生根的总RNA,采用SMAR-Ter cDNA合成技术合成cDNA第1链,优化扩增第2链,通过设置梯度PCR扩增循环数,电泳分析后鉴定cDNA质量。结果表明:未长芽和长芽的材料各1μg总RNA分别成功扩增质量较高的双链cDNA。此方法的应用解决了研究材料有限的问题,可由微量的总RNA扩增出足够多的高质量双链cDNA,为进一步从基因水平研究落地生根奠定基础。Total RNA was extracted from Kalanchoe daigremontiana leaves with or without bulbiferous spurs.The first-strand of cDNA synthesis and the double-strands cDNA amplification were performed using super SMARTer cDNA synthesis technology.The quality of cDNA was evaluated through the gradient of PCR amplification cycles and electrophoresis.High quality double-strands cDNA were obtained from 1 ug total RNA.This method can solve the problem of limited research materials as sufficient high-quality double-strands cDNA are obtained from small amounts of total RNA.This technique allows further research of Kalanchoe daigremontiana.

关 键 词：RNA SMARTer CDNA合成 落地生根 

分 类 号：Q52[生物学—生物化学]