FMLP诱导的嗜中性白细胞呼吸爆发与凋亡的关系研究  

THE RELATIONSHIP BETWEEN fMLP INDUCED NEUTROPHIL RESPIRATORY BURST AND THE APOPTOSIS OF NEUTROPHIL

作　　者：胡天惠[1] 贝泠[1] 黄亚非 沈恂[1] 

机构地区：[1]中国科学院生物物理研究所,北京100101

出　　处：《实验生物学报》1999年第4期359-366,共8页Acta Biologiae Experimentalis Sinica

基　　金：国家自然科学基金(批准号39670205)~~

摘　　要：本文对fMLP诱导的嗜中性白细胞胞内钙浓度变化与凋亡的关系进行了研究。用膜受体激动剂fMLP和钙离子载体A23187诱导细胞内钙浓度升高,BAPTA螯合胞质钙。运用荧光显微镜,流式细胞仪,电泳等方法对培养细胞的凋亡百分率及细胞骨架变化进行了研究。结果表明:fMLP和A23187均有效地抑制了凋亡,而BAPTA促进了凋亡。对骨架测定表明随细胞凋亡微丝解聚明显,胞内钙升高抑制骨架解聚,胞内钙降低促进其解聚。故嗜中性白细胞凋亡过程中伴随有微丝的解聚,胞内钙浓度升高时凋亡被有效抑制,胞内钙浓度降低时促进了凋亡。The relationship between apoptosis of neu-trophils and the change of their intracellular free Ca2+ concentration [Ca2+]i was studied. FMLP and A23187 were used to elevate the [Ca2+]i while BAPTA was used to deplete it. Fluorescence microscope, flow cytometry and gel elec-trophoresis were used to study the percentage of cell apoptosis and the change of f-actin during apoptosis. The results showed that the apoptosis was obviously inhibited by fMLP and A23187, while accelerated by BAPTA. The detection of f-actin showed that the f-actin depolymerized obviously during apoptosis. The elevation of [Ca2+]i inhibit the actin depolymerization while depletion of [Ca2+]i accelerated it. This result indicated that the apoptosis of neutrophil was obviously inhibited by [Ca2+]i elevation but accelerated by [Ca2+]i depletion.

关 键 词：FMLP 嗜中性白细胞 细胞凋亡 呼吸爆发 Ca2+浓度 

分 类 号：Q272[生物学—细胞生物学] Q251