油桐尺蠖单粒包埋核型多角体病毒(BusuNPV)BamHI-H片段的序列分析  

Genetic Organization of the BamHI-H Region of the Single Nucleocapsid Nucleopolyhedrovirus of Buzura suppressaria (BusuNPV )

作　　者：罗保君[1] 王华林[1] 陈新文[1] 孙修炼[1] 王汉中[1] 彭辉银[1] 胡志红[1] BasilM.Arif JustM.Valk 

机构地区：[1]中国科学院武汉病毒研究所中荷无脊椎动物病毒学联合实验室,武汉430071 [2]LaboratopryforMolecularVirology.GreatLakesForestResearchCentre,SaultSte.Marie,on.P6A5M7.Canada [3]DepartmentofVirology,WageningenAgriculturalUniversity,Binnenhaven11,6709PDWageningen,theNetherlands

出　　处：《中国病毒学》1999年第4期333-342,共10页Virologica Sinica

基　　金：国家自然科学基金!3937003;中国科学院与荷兰皇家科学院国际合作课题;中国科学院院长基金

摘　　要：对油桐尺蠖单粒包埋核型多用体病毒（Buzurasuppressariasingle－nucleocapsidnucleopolyhedrovirus，BusuNPV）基因组中BamHI－H片段的序列进行分析，该片段全长2422bp，包括三个开放阅读框：p47基因（AcMNPVORF40的同源区）的5′端，完整的组织蛋白酶基因（cathepsin）（AcMNPVORF127的同源区）和p74基因（AcMNPVORF138的同源区）的3′端。序列比较分析表明，BusuNPV的这三个基因与其它杆状病毒的同源基因具有相同的结构保守区。BusuNPV基因组BamHI－H片段上这三个基因的排列顺序完全不同于AcMNPV相应基因的排列顺序。In order to investigate the genomic organization of the single-nucleocapsid nucleopolyhedrovirus (SNPV) of Buzura suppressaria (BusuNPV), the BamHI-H fragment located at mapunits (mu)81. 6-83. 6 of the viral genome was sequenced. The fragment contained two partialand one complete open reading frames (ORFs) representing the 5' end of a p47 gene, a cathepsingene and the 3' end of a p74 gene. Sequence analysis futher revealed that these ORFs have thesame conserved motifs and gene structure as those observed in their homologues from other baculoviruses. The genomic arrangement of the ORFs in the BusuNPV BamHI-H fragment is verydifferent from the arrangement of their homologues in the genome of Autographa californica mutiple nucleocapsid (M) NPV.

关 键 词：BusuNPV BamHI-H片段 基因 序列分析 

分 类 号：Q939.440.1[生物学—微生物学]