核型多角体病毒的增值与棉铃虫血淋巴酯酶的变化  

The Multiplication of Nuclear Polyhedrosis Viruses and Changes of Hemolymph Esterases in Helicoverpa armigera Larvaes after Infection with NPV

作　　者：徐旭士[1] 赵贵军[2] 李飞[1] 刘润忠[3] 张友清[3] 

机构地区：[1]南昌大学生物学系 [2]中山大学生物防治国家重点实验室,广州510275 [3]中国科学院武汉病毒研究所,武汉430071

出　　处：《中国病毒学》1999年第4期364-367,共4页Virologica Sinica

基　　金：江西省自然科学基金

摘　　要：By the polyacrylamide gel electrophoresis (PAGE) and thin-layer scanning, the esterases (EST) of the third instar larvae of the Helicoverpa armigera were investigated in qualityand quantity after infection with nuclear polyhedrosis viruses (NPV). The changes in sort andcontent of hemolymph EST were examined at six hours postinfection and the numbers of ESTbands were from more to less as develop and infective time in larvae. The varies in EST patternbetween infection and control were not significant but the content of each band and the accumulation of EST were obvious. According to the changeable regularity in EST content of zymogram,the total and single content in diseased larvaes always gradually decreased with passages of infective time but in the healthy larvaes had no distinct changes. The results demonstrated that the infection with NPV could affect the EST metabolism in larvae hemolymph, and the metabolicchanges may show the infective level and extent, there fore it had indicated function.By the polyacrylamide gel electrophoresis (PAGE) and thin-layer scanning, the esterases (EST) of the third instar larvae of the Helicoverpa armigera were investigated in qualityand quantity after infection with nuclear polyhedrosis viruses (NPV). The changes in sort andcontent of hemolymph EST were examined at six hours postinfection and the numbers of ESTbands were from more to less as develop and infective time in larvae. The varies in EST patternbetween infection and control were not significant but the content of each band and the accumulation of EST were obvious. According to the changeable regularity in EST content of zymogram,the total and single content in diseased larvaes always gradually decreased with passages of infective time but in the healthy larvaes had no distinct changes. The results demonstrated that the infection with NPV could affect the EST metabolism in larvae hemolymph, and the metabolicchanges may show the infective level and extent, there fore it had indicated function.

关 键 词：棉铃虫 血淋巴 酯酶 核型多角体病毒 EST 

分 类 号：S435.622.2[农业科学—农业昆虫与害虫防治] S476.13[农业科学—植物保护]