30日龄Fmr1基因敲除小鼠的跳台实验观察*  

Behavioural Comparison of Fmr1 Knockout Mice at 30 Days Age in Step-down Test

作　　者：黄月玲[1] 沈岩松[2] 张维雯[3] 孙卫文[2] 李敏雄[2] 陈盛强[2] 戴丽军[1] 

机构地区：[1]广州医学院实验动物中心,广州510182 [2]广州医学院第二附属医院,广州510260 [3]广州医学院第三附属医院,广州510340

出　　处：《实验动物科学》2011年第5期3-6,共4页Laboratory Animal Science

基　　金：广东省自然科学基金项目(No.815101700100005);广东省科技计划项目(No.2005B60302004)(2008B030301371)(2009B030801368)(2009B060300018);广东省中医药管理局建设中医药强省科研基金项目(No.2008184);广州医学院科研立项项目(04-k-53);广州市中医药中西医结合科研立项项目(No.2008A52)

摘　　要：目的对30日龄的Fmr1基因敲除小鼠的跳台实验进行观察。方法采用30日龄的KO鼠和WT鼠分别连续进行2 d的跳台实验,根据所获得的数据进行多因素方差分析处理。结果同周龄KO鼠的潜伏期比WT鼠明显少(P<0.05);而KO鼠的错误次数比WT鼠明显多(P<0.05);不同周龄KO鼠或WT鼠的潜伏期、错误次数无差异(P>0.05);第1天KO鼠的潜伏期和错误次数与第2天相比无差异(P>0.05);第1天WT鼠的潜伏期和错误次数与第2天相比有显著差异(P<0.05)。结论 30日龄Fmr1基因敲除小鼠存在认知功能障碍。Objective This study was designed to observe the cognition of Fmr1 knockout mice at 30 days Age in step-down test.Method Fmr1 knockout mice were identified using the PCR technical and step-down test were used in the study.Animals were tested for two days.The latency and the number of errors were recorded.The data was analyzed with multifactor variance analysis.Result KO mice obviously had the shorter latency than WT mice,and KO mice obviously had more errors than WT mice（P0.05）;On the first day,the latency and the number of errors of KO mice had no difference compared with the second day（P0.05）;On the first day,the latency and number of errors of WT mice had significant difference compared with the second day（P0.05）.Conclusion Fmr1 knockout mice displayed cognitive impairment in the step-down test.

关 键 词：跳台实验 FMR1 基因敲除 小鼠 认知 

分 类 号：Q95-331[生物学—动物学]