花生根瘤菌的数值分类  

NUMERICAL TAXONOMY OF BRADYRHIZOBIA ISOLATED FROM PEANUT NODULES

作　　者：曹凤明[1] 徐玲玫[1] 李力[1] 李俊[1] 樊蕙[1] 

机构地区：[1]中国农业科学院土壤肥料研究所,北京100081

出　　处：《微生物学通报》1999年第6期421-425,共5页Microbiology China

基　　金：欧盟合作课题资助!(NO.ERB3514PL950967);E. C FUNDED PROJECT(NO;ERB3514PL950

摘　　要：从我国 １１个省、 １６种土壤类型、 ２０多个花生（Ａｒａｃｈｉｓ ｈｙｐｏｇａｅａ Ｌ．）品种上分离到的花生根瘤菌［Ｂｒａｄｙｒｈｉｚｏｂｉｕｍ ｓｐ．（Ａｒａｃｈｉｓ）］中选取５０个代表菌株、并与从津巴布韦引进的４株花生根瘤菌及７株慢生根瘤菌常用的参比菌株共６１株菌进行了１２８项表型特征的测定。数值分类的结果表明，全部菌株在７５％水平上相聚，并在７６％和７７％的水平上分别聚成两大群（群Ⅰ、群Ⅱ），每大群以较高的相似性（８５％- ９４％）各聚成６个亚群。所用数值分类方法不能将花生根瘤菌和大豆根瘤菌在属的水平上分开，但亚群和未归入亚群的菌株可能暗示亚种或种存在，同时本文结果还表现出花生根瘤菌的地域分布差异及其多样性。50 strains of peanut rhizobia [Bradyrhizobium sp. (Arachis)], which were isolated from root nodules of over 20 peanut cultivars in 16 types of soil in 11 provinces of China were compared with 4 strains of peanut rhizobia from Zimbabwe and 7 strains known species of Bradyrhizobium (B. liaoningense, B japonicum, B elkanii) by performing a numerical analysis of 128 phenotypic features. The result showed that all of the strains were clustered at the similarity level of 75%, and groupI and groupⅡ were gathered at the level of 76% and 77%, respectively. Each group further was respectively separated into 6 subgroups at the level of 85% and 87%. The result suggested that peanut rhizobia from china could not be differentiated from the three known species of Bradyrhizobium, but there probably existed new species or subspecies in the subgroups and those strains not belonging to any subgroup, and existed significant genetic diversity.

关 键 词：花生根瘤菌 数值分类 多样性 根瘤菌分类 

分 类 号：Q939.114[生物学—微生物学]