WRKY25过量表达导致拟南芥在长光照下开花提前  

Overexpression of WRKY25Causes Early Flowering in Arabidopsis under Long-day Conditions

作　　者：王芳秀[1,2] 黎舒佳[1,3] 余迪求[1] 

机构地区：[1]中国科学院西双版纳热带植物园,云南昆明650223 [2]中国科学院研究生院,北京100049 [3]中国科学院遗传与发育生物学研究所,北京100101

出　　处：《植物分类与资源学报》2011年第6期653-659,共7页Plant Diversity

摘　　要：WRKY基因家族是主要存在于植物中的一大类转录调控因子,拥有很多家族成员。拟南芥WRKY25属于第I类WRKY蛋白,参与植物生物和非生物胁迫反应。通过GUS染色和qRT-PCR发现WRKY25基因主要在根,叶和茎生叶中表达。过量表达WRKY25的转基因植株在长光照下比野生型拟南芥提前开花。通过RT-PCR检测与开花时间相关基因发现,AP1基因的表达量在培养21d和27d的WRKY25过量表达植株中上调,由此推测WRKY25很可能通过增强AP1的表达来影响开花。The WRKY gene superfamily is one of the largest transcriptional regulators which mainly exists in plants. Arabidopsis WRKY25 is one of the group I WRKY proteins and responds to both biotic and abiotie stresses. GUS staining and qRT-PCR analysis show that the expression of WRKY25 mainly focuses on roots, rosette leaves and eau- line leaves. Transgenic plants over-expressing WRKY25 show early flowering under long-day conditions when com- pared with wild-type plants. RT-PCR analysis demonstrates that the expression of AP1 is up-regulated in 21 d-old and 27 d-old transgenie plants. Thus, it is possible that WRKY25 affects flowering through up-regulation of AP1 di- rectly or indirectly.

关 键 词：WRKY25 过量表达 长光照 AP1 提前开花 

分 类 号：Q945[生物学—植物学]