抗松针褐斑病湿地松组培苗生根条件的优化  

Optimization of root formation in microcuttings of disease-risistant Pinus elliottii

作　　者：安会翠[1] 叶建仁[1] 吴小芹[1] 刘戈[1] 朱丽华[1] 

机构地区：[1]南京林业大学森林资源与环境学院,江苏省有害生物入侵预防与控制重点实验室,江苏南京210037

出　　处：《南京林业大学学报（自然科学版）》2011年第6期59-62,共4页Journal of Nanjing Forestry University：Natural Sciences Edition

基　　金：国家林业局“948”项目(2011-4-69);江苏高校优势学科建设工程资助项目

摘　　要：以抗松针褐斑病湿地松组培苗为研究材料,对影响组培苗不定根发生的条件进行优化。结果表明:在含4.0 mg/L IBA和0.07 mg/L NAA的WPM培养基中添加0.4 mg/L多效唑对湿地松组培苗不定根的形成和再生植株的成活率有促进作用;湿地松组培苗不定根原基形成后,出瓶移至装有蛭石的穴盘中,有利于不定根的表达,且根茎连接非常好,植株成活率高达85%;基因型对苗的成活率有决定性的影响,不同家系及同一家系不同无性系间移栽成活率差异较大。The conditions for root formation and development in microcuttings of Pinus elliottii which were resistant to Lecanosticta acicola were optimized.The results showed that the addition of 0.4 mg/L PP333 to WPM medium in combination with 4.0 mg/L IBA and 0.07 mg/L NAA was beneficial to root induction phase.After the formation of adventitious root primordia,the microcuttings were transferred into the disk containing vermiculite,which were favorable to root expression.Using this procedure,the frequency of root induction was up to 100 % and the survival rate of regerated plantlets reached up to 85 %.Significant genotypic variation at both the family and the clone-within-family levels was observed in the survival rate of transplanted plantlets.

关 键 词：抗性湿地松 生根 再生植株 成活率 

分 类 号：S432.21[农业科学—植物病理学]