玉米中哺乳动物细胞凋亡相关基因rb同源序列的检出及其荧光原位杂交  

Detection and Fluorescence in Situ Hybridization of Homologous Sequences for Mammalian Gene rb Related to Apoptosis in Maize

作　　者：杨征[1] 蔡陈崚 刘立华[1] 宋运淳[1] 

机构地区：[1]武汉大学生命科学学院发育生物学中心,武汉430072

出　　处：《Acta Botanica Sinica》1999年第12期1339-1341,共3页Acta Botanica Sinica（植物学报：英文版）

摘　　要：HumangenerbrelatedtoapoptosiswasusedastheprobefortheSouthernblothybridizationofthe genomicDNAinbothmaize (ZeamaysL .)andrice (OryzasativaL .) .Theresultsindicatedthattheho mologoussequencesofrbwerepresentedinthetwospecies .Thephysicallocationoftherbhomologousse quenceswasalsocarriedoutinmaizechromosomesbyfluorescenceinsituhybridization (FISH) .Thegenerb washybridizedontothelongarmsofthechromosomes 5and 6 ,andtheshortarmofthechromosome 8.The detectionratesofFISHwere 7.58% ,16 .16 %and 10 .10 % ,andpercentdistancesfromcentromeretothe detectionsiteswere 86 .17± 3.2 2 ,94 .10± 2 .59and 92 .4 7± 2 .33respectively .Theseresultsprovidedim portantcluestofurtherresearchofplantapoptosisgenes .HumangenerbrelatedtoapoptosiswasusedastheprobefortheSouthernblothybridizationofthe genomicDNAinbothmaize (ZeamaysL .)andrice (OryzasativaL .) .Theresultsindicatedthattheho mologoussequencesofrbwerepresentedinthetwospecies .Thephysicallocationoftherbhomologousse quenceswasalsocarriedoutinmaizechromosomesbyfluorescenceinsituhybridization (FISH) .Thegenerb washybridizedontothelongarmsofthechromosomes 5and 6 ,andtheshortarmofthechromosome 8.The detectionratesofFISHwere 7.58% ,16 .16 %and 10 .10 % ,andpercentdistancesfromcentromeretothe detectionsiteswere 86 .17± 3.2 2 ,94 .10± 2 .59and 92 .4 7± 2 .33respectively .Theseresultsprovidedim portantcluestofurtherresearchofplantapoptosisgenes .

关 键 词：玉米 细胞凋亡 RB 同源序列 荧光原位杂交 

分 类 号：Q949.714.2[生物学—植物学] Q343.2