犬呼肠病毒3型感染BHK-21细胞的超微结构观察  

Ultrastructural Featrues of Canine Reovirus Type 3 Infection in BHK-21 Cell Cultures

作　　者：遇秀玲[1] 田克恭[1] 沈永舟 杨怡[3] 汪宝珍[3] 吴娜[1] 杨汉春[4] 

机构地区：[1]军事医学科学院实验动物中心,北京100071 [2]安徽农业大学 [3]军事医学科学院仪器中心 [4]中国农业大学

出　　处：《实验动物科学与管理》1999年第4期26-28,共3页Laboratory Animal Science & Administration

摘　　要：本实验采用免疫电镜负染、细胞超薄切片透射电镜观察 ,证实所分离的病毒为犬呼肠病毒。病毒粒子由致密的核心和包围着核心的双层衣壳组成 ,有的为空心衣壳 ,多呈晶格状排列 ;病毒在胞浆增殖 ,装配 ,通过细胞崩解释放 ;细胞浆内可见大量的微丝与微管结构 ,认为与病毒复制有关。胞浆与胞核中均可见到内容物不同 。? Canine Reovirus type 3,designated Reo-3 AMMS,originally obtained from a dog with respiratory signs and diarrhea in China,was further confirmed by electron microscope with ultrathin sectioning and phosphotungstic acid negative staining methods.The Reo-3 virions composed of dense core and two layered capsids aggeregate to crystalline arrangement.The Reo-3 replicates in cytoplasm and releases from disrupted cells.It showed that bodies of different contents and in varying forms were seen in nucleus and cytoplasm.A large amount of filementous and tubular structures observed in cytoplasm were deemed to be relevant to viral replication.

关 键 词：犬 犬呼肠病毒 BHK-21细胞 超微结构 致病机理 

分 类 号：S858.292.5[农业科学—临床兽医学] S852.655[农业科学—兽医学]