Laccase biosensor using magnetic multiwalled carbon nanotubes and chitosan/silica hybrid membrane modified magnetic carbon paste electrode  

作　　者：庞娅 曾光明 汤琳 章毅 李贞 陈丽娟 

机构地区：[1]College of Environmental Science and Engineering, Hunan University [2]Key Laboratory of Environmental Biology and Pollution Control of Ministry of Education, Hunan University

出　　处：《Journal of Central South University》2011年第6期1849-1856,共8页中南大学学报（英文版）

基　　金：Project(IRT0719) supported by the Program for Changjiang Scholars and Innovative Research Team in University, China;Projects (50978088, 51039001) supported by the National Natural Science Foundation of China;Project(2009FJ1010) supported by the Hunan Key Scientific Research Program, China;Project(10JJ7005) supported by the Natural Science Foundation of Hunan Province, China;Projects(CX2009B080, CX2010B157) supported by the Hunan Provincial Innovation Foundation For Postgraduate;Project supported by the Fundamental Research Funds for the Central Universities, Hunan University, China

摘　　要：A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes (MMCNT) which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope (SEM) and vibrating sample magnetometer (VSM) were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica (CS) hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10-7 to 0.165×10-3 mol/L. The corresponding detection limit is 3.34×10-8 mol/L based on signal-to-noise ratios (S/N) ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.A simple and rapid strategy to construct laccase biosensor for determination of catechol was investigated. Magnetic multiwalled carbon nanotubes （MMCNT） which possess excellent capability of electron transfer were prepared by chemical coprecipitation method. Scanning electron microscope （SEM） and vibrating sample magnetometer （VSM） were used to identify its surfacetopography and magnetization, respectively. Laccase was immobilized on the MMCNT modified magnetic carbon paste electrode by the aid of chitosan/silica （CS） hybrid membrane. Using current-time detection method, the biosensor shows a linear response related to the concentration of catechol in the range from 10-7 to 0.165×10-3 mol/L. The corresponding detection limit is 3.34× 10-8 mol/L based on signal-to-noise ratios （S/N） ≥3 under the optimized conditions. In addition, its response current retains 90% of the original after being stored for 45 d. The results indicate that this proposed strategy can be expected to develop other enzyme-based biosensors.

关 键 词：magnetic multiwalled carbon nanotubes PARAMAGNETISM chitosan/silica sol laccase biosensor catechol 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]