黄芩苷对流感病毒FM1肺炎小鼠肺损伤的保护研究  

Protection effect of baicalin on lung injury of mice infected with influenza FM1

作　　者：万巧凤[1,2] 顾立刚[1] 殷胜骏[1] 祈广见[1] 张舒[1] 李根茂[1] 葛东宇[1] 

机构地区：[1]北京中医药大学中医药抗病毒教育部重点实验室,北京100029 [2]宁夏医科大学,银川750004

出　　处：《中华中医药杂志》2011年第12期2848-2851,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.30772872)~~

摘　　要：目的:研究黄芩苷对流感病毒FM1感染小鼠肺组织NO及氧化损伤的保护作用。方法:将96只ICR鼠随机分为正常对照组、模型组、利巴韦林组(100mg.kg-1.d-1),黄芩苷大剂量组(1 500mg.kg-1.d-1),黄芩苷中剂量组(750mg.kg-1.d-1)和黄芩苷小剂量组(375mg.kg-1.d-1),每组16只。用流感病毒亚甲型鼠肺适应株A/FM/1/47(H1N1)感染小鼠,建立小鼠流感病毒性肺炎的生物模型,用不同剂量黄芩苷溶液灌胃治疗后,观察小鼠肺指数、肺组织H-E染色切片病理形态变化,比色法检测肺组织匀浆总NO合成酶(NOS)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-PX)含量或活性。结果:黄芩苷中、小剂量组均能明显降低肺指数,减轻肺组织病变;显著下调肺匀浆总NO合成酶、丙二醛及上调谷胱甘肽过氧化物酶含量或活性(P<0.05,P<0.01)。结论:黄芩苷可通过抑制流感病毒性肺炎小鼠肺组织NO及过氧化物水平而发挥保护作用。Objective： To investigate the protective function of baicalin on lung NO and oxidized damages to influenza virus infection in mice.Methods： 96ICR mice were randomly divided into six groups： normal control,model control,ribavirin（100mg·kg-1·d-2）,baicalin high dose（1 500mg·kg-1·d-2）,baicalin middle dose（750mg·kg-1·d-2） and baicalin low dose（375mg· kg-1·d-2）,16 rats in each group.The pneumonia model was established by infection of influenza virus strain A/FM/1/47（H1N1）.Model mice were treated with different dose group of baicalin by intragastric administration.The contents of NOS,MDA and GSH-PX was detected by color methods.At the same time,observing the pathological change of lung injury.Results： middle and low dose groups of baicalin decreased the contents of NOS and MDA,increased the contents of GSH-PX obviously（P0.05,P0.01）,reduced lung index,alleviated the pathological change of lung injury significantly.Conclusion： Baicalin has an obviously protective effect by decreasing the level of NO and peroxides in the lungs of mice infected with influenza virus FM1.

关 键 词：黄芩苷 流感病毒 肺损伤 NO合成酶 丙二醛 谷胱甘肽过氧化物酶 

分 类 号：R285.5[医药卫生—中药学]