钠钾氯同向转运体2在新生小鼠延髓脑片基本节律性呼吸放电产生中的作用  

Roles of Na-K-2Cl cotransporter 2 on respiratory-related rhythmic discharge activity in brainstem slice in neonatal mice

作　　者：付素珍[1] 千智斌[2] 刘友才[2] 姬明丽[2] 千新来[2] 

机构地区：[1]新乡医学院第一附属医院儿科,河南卫辉453100 [2]新乡医学院基础医学院

出　　处：《中华脑科疾病与康复杂志（电子版）》2011年第1期9-11,共3页Chinese Journal of Brain Diseases and Rehabilitation（Electronic Edition）

基　　金：河南省科技创新人才计划科技创新杰出青年基金(094100510007);河南省科技攻关项目(102102310156)

摘　　要：目的研究延髓面神经后核内侧区呼吸神经元钠钾氯同向转运体（NKCC2）对基本节律性呼吸的调控作用。方法制作包含面神经后核内侧区（mNRF）并保留舌下神经根的新生小鼠离体延髓脑片标本，灌流改良Kreb液（MKS），使用吸附电极记录其舌下神经根的呼吸相关节律性放电活动（RRDA）。依次使用含NKCC2阻断剂呋塞米0、0．125、0．250、0．500和1．000mmol／L的MKS灌流脑片，以呼吸周期（RC）、吸气时程（TI）和放电幅度积分（IA）为观察指标，研究阻断呼吸神经元细胞膜上NKCC2对RRDA的影响。结果在0—0．500mmol／L浓度范围内，呋塞米可缩短RRDA的RC（P〈0．05或0．01），且呈浓度依赖性，1．000mmol／L的呋塞米延长RC（F=25．623，P〈0．05）。随呋塞米浓度增加RRDA的TI逐渐延长（F=6．063，P〈0．05）。在0—0．500mmol／L浓度范围内，RRDA的IA随呋塞米浓度增加而增加（P〈0．05），1．000mmol／L的呋塞米可降低IA（F=6．778，P〈0．05）。在0～0．500mmol／L浓度范围内，呋塞米对RRDA有兴奋作用，1．000mmol／L的呋塞米对RRDA有抑制作用。结论延髓mNRF神经元细胞膜上存在NKCC2，参与新生小鼠延髓基本节律性呼吸的调控。Objective To investigate the roles of Na-K-2Cl eotransporter 2 （NKCC2） on respiratory-related rhythmic discharge activity（RRDA） in neonatal mice. Methods Experiments were performed on in vitro neonatal mice brainstem slices. The slices containing the medial region of the nucleus retrofacialis（mNRF） with the hypoglossal nerve rootlets retained were prepared. RRDA were recorded frmn hypoglossal nerve rootlets by suction electrode. The possible roles of NKCC2 on RRDA were investigated by administration of furosemide, a blocking agent of NKCC2, dissolved in modified Kreb＇s solution（MKS） for perfusion of the brainstem slices. Six slices positive of RRDA were perfused with different concentrations （0, 0. 125, 0. 250, 0. 500 and 1. 000 mmol/L）of furosemide. The respiratory cycle （RC） , inspiratory time （TI） and integral amplitude （IA） were adopted as observation indexes. Results At the concentration range of 0-0.500 retool/L, furosemide shortened RC （P 〈 0.05 or 0.01 ） , 1. 000 mmo]/L furosemide prolonged RC （ F = 25. 623, P 〈 0.05 ）. At the concentration of 0-1. 000 mmol/L furosemide prolonged TI （F = 6. 063,P 〈 0.05 ）. Furosemide enhanced IA at 0. 250 mmoL/L and 0. 500 mmol/L（ P 〈 0.05 ）, and degraded IA at 1. 000 retool/L（ F = 6.778 ,P 〈 0. 05 ）. Furosemide had a exciting role on RRDA at the concentration range of 0- 0. 500 retool/L, and had a rejection capability role at 1. 000 mmol/L. Conclusions NKCC2 takes part in modulation on the RRDA of brainstem slices in neonatal rats.

关 键 词：小鼠 新生 钠钾氯同向转运体2 节律性呼吸放电 延髓脑片 

分 类 号：R722.1[医药卫生—儿科]