碳源对山核桃体细胞胚发生和植株再生的影响  

Somatic embryogenesis and plant regeneration from Carya cathayensis embryos using different carbon sources

作　　者：胡恒康[1,2,3] 江香梅[4] 张启香[1,3] 陈贝[1,3] 黄坚钦[1,3] 

机构地区：[1]浙江农林大学林业与生物技术学院,浙江临安311300 [2]江西农业大学园林与艺术学院,江西南昌330045 [3]浙江农林大学亚热带森林培育国家重点实验室培育基地,浙江临安311300 [4]江西省林业科学院,江西南昌330032

出　　处：《浙江农林大学学报》2011年第6期911-917,共7页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金重大项目(Z307534);浙江省自然科学基金资助项目(Y3090091);浙江省科学技术攻关项目(2009C32027);浙江农林大学B类团队创新项目(2007);浙江省大学生科技创新项目(2008)

摘　　要：以山核桃Carya cathayensis花后10～12周的幼胚为外植体,建立体胚发生及再生体系,同时分别对山核桃胚性愈合组织和体细胞胚诱导的碳源种类和质量浓度进行了比较分析。结果表明:接种在含葡萄糖培养基中的山核桃胚性愈合组织诱导率显著高于蔗糖、海藻糖和麦芽糖,诱导率达33.3%;在培养基中添加20 g.L-1葡萄糖,胚性愈合组织诱导率最高,达50.0%,显著高于其他处理。蔗糖为山核桃体细胞胚诱导的最佳碳源种类,体细胞胚诱导率显著高于葡萄糖、海藻糖和麦芽糖,诱导率达23.3%;在培养基中添加45 g.L-1蔗糖,体细胞胚诱导率最高,达30.1%,显著高于其他处理。体胚在WPM培养基(木本植物用培养基)附加5 g.L-1蔗糖的培养基上萌发率达20%,显著高于附加0,10,15,20,25,30 g.L-1蔗糖的体胚萌发率。A process for studying somatic embryogenesis and plant regeneration in vitro from immature embryos of Carya cathayensis,which were 10-12 weeks post-pollination,was developed.To select a carbon source（glucose,sucrose,trehalose,and maltose） for embryonic callus,a single-factor experimental design was used.Then,the optimal concentration of glucose（10,20,40,60 and 80 g·L-1） was determined.Also,for somatic embryogenesis,the best carbon source was decided.Then,the optimal sucrose concentrations（15,30,45,60 and 75 g·L-1） for somatic embryogenesis and for somatic embryo germination were determined.Results showed that the embryogenic callus induction rate of immature embryos inoculated in the medium supplemented with glucose was significantly higher（P 0.05） than sucrose,trehalose,and maltose.Embryogenic callus percentage was significantly greater（P 0.05） with 20 g·L-1 glucose with a 50.0%.For somatic embryogenesis of immature embryos,sucrose as a carbon source was significantly better（P 0.05） than glucose,trehalose,and maltose.Somatic embryogenesis induction was significantly higher（P 0.05） with 45 g·L-1 sucrose.For somatic embryo germination,a basal woody plant medium（WPM） supplemented with 5 g·L-1 sucrose was significantly higher（P 0.05） than other concentrations.

关 键 词：经济林学 山核桃 碳源 体细胞胚 植株再生 

分 类 号：S718.4[农业科学—林学]