L20B RD与Hep-2三种细胞用于脊髓灰质炎病毒分离鉴定的比较  

Comparison among L20B,RD and Hep-2 cells applied to isolate and identify polioviruses

作　　者：潘伟毅[1] 蔡志坤[1] 杨莹[1] 何亚玲[1] 

机构地区：[1]福建省卫生防疫站,福建福州350001

出　　处：《中国计划免疫》1999年第6期324-326,329,共4页Chinese Journal of Vaccines and Immunization

摘　　要：采用世界卫生组织（ＷＨＯ） 推荐使用的方法，用Ｌ２０Ｂ、ＲＤ和Ｈｅｐ２ 三种细胞进行脊髓灰质炎（脊灰）病毒的分离鉴定。结果表明，Ｌ２０Ｂ细胞具有良好的特异性，对非脊灰肠道病毒均不敏感。在脊灰分离株和粪便悬液标本中脊灰病毒的检出率分别为１００％ 和９３－３ ％ ，优于ＲＤ和Ｈｅｐ２ 细胞，特别是在病毒分布不均及混有非脊灰肠道病毒的情况下，能更好地、准确地捕捉到脊灰病毒。采用Ｌ２０Ｂ加ＲＤ细胞分离脊灰病毒优于ＲＤ加Ｈｅｐ２，但对非脊灰肠道病毒分离率有影响。We adopted the method recommended by WHO to isolate and identify polioviruses with L20B, RD and Hep 2 cells. The results showed that L20B cells has good specificity, and is not sensitive to non polio enteroviruses. For isolating poliovirus of known strains and from stool suspension specimens the poliovirus detection rates were 100% and 93.3% separately, all higher than using RD and Hep-2 cells. Especially in the case that poliovruses distributed not evenly or mixed with non-polio enteroviruses, L20B cells can catch polioviruses from specimen efficiently and accurately. Adopting L20B and RD cells to isolate and identify polioviruses is superior to RD and Hep-2 cells, but for non-polio enteroviruses the isolation rate would be affected.

关 键 词：脊髓灰质炎 病毒 分离率 L20B RD HEP-2 

分 类 号：R512.404[医药卫生—内科学] R373.22[医药卫生—临床医学]