ILK在非慢性排斥CAN患者移植肾组织的表达及其意义  

作　　者：梁桂荣[1] 晏强[2] 邹和群[3] 眭维国[2] 刘作海[2] 罗皓[1] 

机构地区：[1]南方医科大学,510515 [2]南方医科大学附属桂林181医院全军肾移植与透析中心,541002 [3]南方医科大学附属三院肾内科,泌尿疾病研究所,510630

出　　处：《浙江临床医学》2011年第12期1321-1324,共4页Zhejiang Clinical Medical Journal

摘　　要：目的探讨非慢性排斥CAN（慢性移植肾病）患者移植肾组织中整合素连接激酶（ILK）、转化生长因子β1（TGF-β1）的表达，及与移植肾间质纤维化／肾小管萎缩（IF／TA）的关系。方法用免疫组织化学技术和计算机真彩色图像分析系统半定量检测48例非慢性排斥CAN患者移植肾组织中ILK和TGF—β1的表达情况，分析二者间及与非慢性排斥CAN患者移植肾IF／TA病理分级之间的关系。15例正常肾组织作为对照。结果非慢性排斥CAN各组间的移植肾组织中ILK、TGF-β1的表达比正常肾组织明显增加（P〈0．01），并随IF／TA病理分级呈逐渐递增的趋势；非慢性排斥CAN移植肾组织中ILK的表达与TGF-β1呈正相关（r=0．930，P〈0．01）；ILK、TGF-β1表达水平分别与非慢性排斥CAN移植肾IF／TA病理分级呈正相关，（r=0．860、0．938；P〈0．01）；Scr与IF／TA病理分级呈正相关（r=0．790，P〈0．01）；ILK与SCr之间呈正相关关系（r=0．865，P〈0．001）。结论ILK可能是介导TGF-β1促进非慢性排斥CAN患者移植肾细胞外基质（extra cellular matrix，ECM）异常沉积发病机制，ILK在非慢性排斥CAN患者移植肾纤维化细胞信号通路中起重要作用。Objective To investigate the expression of integrin - linked kinase （ILK） and transforming growth factor - β1 （TGF- β1 ） in the renal allografts with non -chronic rejection CAN （chronic allograft nephropathy） , and explore their correlation with the pathogenesis of IF/TA in renal allograft with non - chronic rejection CAN. Methods Immunohistochemieal assay and computer- assisted genuine colored image analysis system were used to detect the expression of ILK and TGF - β1 in the renal allografts of 48 patients with non - chronic rejection CAN. The correlation between TGF - β1 and ILK, as well as the correlation between their expressions and the pathological grading of IF/TA in renal allograft with non - chronic rejection CAN were analyzed. 15 specimens of healthy human renal tissue were used as controls. Results The expression levels of ILK and TGF -β1 were significantly higher in the renal allograft tissue with non -chronic rejection CAN, compared to normal renal tissue （P 〈0. 01 ） , and the expression levels tended to increase with the increase of the pathological grading of IF/TA. In non - chronic rejection CAN groups, the expression of ILK was positively correlated with TGF- β1 （r = 0. 930 ,P 〈0. 01 ）. In renal allografts with non -chronic rejection CAN, a significant positive correlation was found between either ILK or TGF -β1 expression level and pathological grading of IF/TA （r = 0. 860, 0. 938, P 〈 0. 01 respectively）. There was a positive correlation between Scr level and pathological grading of IF/TA. The expression of ILK was positively correlated with that of Scr （ r = 0. 865, respectively ; P 〈 0.01 ）. Conclusions It is suggested by the data that ILK might mediate the pathogenesis of TGF -β1 - induced abnormal deposition of ECM in renal allograft with non - chronic rejection CAN, and that ILK might play a key role in the signaling pathway of renal fibrosis in renal allogtaft with non- chronic rejection CAN.

关 键 词：整合素连接激酶 慢性移植肾病 移植肾间质纤维化/肾小管萎缩 转化生长因子-Β1 

分 类 号：R699.2[医药卫生—泌尿科学]