反胶团酶促水解青霉素G制备6-APA  

Study on penicillin G hydrolysis to 6-APA in reversed micelles

作　　者：何志敏[1] 郭从容[1] 吴金川[1] 胡朗 

机构地区：[1]天津大学化学工程研究所,天津300072

出　　处：《中国抗生素杂志》1999年第6期404-407,共4页Chinese Journal of Antibiotics

基　　金：天津市自然科学基金!资助(项目编号:97360281)

摘　　要：研究将青霉素酰化酶包埋于ＡＯＴ／异辛烷反胶团中水解青霉素Ｇ制备６－ＡＰＡ的工艺，探讨了各种操作条件对水解过程的影响。结果表明，在反胶团酶反应体系中，底物浓度提高了一倍左右，且在反应过程中不需滴碱调节ｐＨ值。在水相与反胶团体系各自的最适条件下分别进行水解反应实验，结果表明，青霉素酰化酶在反胶团体系中的酶活力高于水相酶活力，水解６ｈ 后其转化率可达７０％ 以上；与相应的水相酶促反应相比，转化率提高了３０％ 。In this paper, the hydrolysis course of penicillin G to 6 APA with the penicillin amidase entrapped in AOT/isooctane reversed micelles was investigated. The effects of reaction conditions such as moisture content (W 0), concentration of AOT, temperature, pH of buffer and concentration of substrate were discussed in detail. It was shown that the concentration of substrate was increased one fold. Furthermore, the pH of reaction systems was almost constant without adjusting it by adding bases. The experiments of penicillin G hydrolysis to 6 APA were carried out in the reversed micelles and the pure buffer under their own appropriate conditions respectively. The results indicated that the conversion of penicillin G hydrolysis was over 70% in AOT/isooctane reversed micelles system after 6h, corresponding to 30% in the pure buffer. As a result, a new and efficient technology for the hydrolysis of penicillin G under the higher concentration of substrate was achieved successfully.

关 键 词：青霉素 6-APA 反胶团酶 水解 

分 类 号：TQ465.1[化学工程—制药化工]