白三叶高频组织培养再生体系的研究  

Optimization of Regeneration System of Trifolium repens L.

作　　者：齐广勋[1,2] 杨向东[2] 隋丽[2] 张正坤[2] 郭东全[2] 钱雪燕[2] 邢国杰[2] 杨静[2] 董英山[2] 王金刚[1] 李启云[2] 

机构地区：[1]东北农业大学园艺学院,哈尔滨150030 [2]吉林省农业科学院,长春130033

出　　处：《吉林农业科学》2011年第4期51-54,共4页Journal of　Jilin Agricultural Sciences

基　　金：国家863计划(2007AA10Z189);国家转基因生物新品种培育重大专项(2008ZX08004-004);国家转基因生物新品种培育重点项目(2008ZX08004-006B)

摘　　要：高频稳定的组织培养再生体系是开展白三叶遗传转化的重要前提。为进一步优化白三叶再生体系,本研究以白三叶子叶节为外植体,对影响丛生芽诱导、增殖的激素种类(2,4-D、KT、6-BA和NAA)和浓度进行了优化;同时研究了不同基质配比对组培苗移栽成活率的影响。结果表明:在含6-BA 0.5 mg.L-1和NAA0.1 mg·L-1诱导培养基上丛生芽诱导率最高,达68.33%,且生长状态最好;在含IBA 0.2 mg·L-1的1/2MS培养基上诱导生根率为90%以上,平均每株生根数达6条以上;移栽至腐殖土和珍珠岩(2:1)混合基质中的再生苗成活率可达85%。本研究结果可为建立豆科植物转化体系、加快白三叶基因工程改良及创制白三叶新种质奠定基础。High-frequent regeneration is essential for plant genetic transformation.To further improve regeneration efficacy of white clover（Trifolium repens L.）,different concentration and combination of phytohormones of 2,4-D,KT,6-BA and NAA were investigated on shoots induction and proliferation from the cotyledon.And acclimation of the regenerated plantlets was also optimized.The results showed that better-quality of the shoots cluster can be induced from the cotyledon when the explants grew on the MS induction medium supplemented with 0.5 mg·L-1 6-BA and 0.1 mg·L-1 NAA.Besides,about 90% of shoots could take roots with an average of more 6 adventitious roots for every green shoot after four-weeks culture on 1/2MS medium containing 0.2 mg·L-1 IBA.Moreover,over 85% of the regenerated plantlets survived when transplanted on the mixture of the turf and pearlite（2：1）.Our progress will do good help to genetic transformation system of Leguminosae plants,genetic improvement and germplasm innovation of Trifolium repens L.

关 键 词：白三叶 子叶节 组织培养 再生体系 

分 类 号：S541.203.53[农业科学—作物学]