酶消化法分离老龄SD大鼠血管平滑肌细胞  

A method of enzyme digestion for dissociation of vascular smooth muscle cells in aged SD rats

作　　者：王明勇[1] 陈枫[1] 陈庄[1] 

机构地区：[1]泸州医学院附属医院医学实验中心,四川泸州646000

出　　处：《重庆医学》2011年第34期3482-3483,共2页Chongqing medicine

摘　　要：目的采用酶消化法培养老龄SD大鼠血管平滑肌细胞,为血管疾病的研究提供大量的原代平滑肌细胞。方法无菌取老龄SD大鼠主动脉,采用0.2%Ⅱ型胶原酶消化分离血管平滑肌细胞,自然纯化及差速贴壁纯化血管平滑肌细胞,免疫组化鉴定平滑肌细胞α肌动蛋白。结果免疫组化染色显示细胞纯度在95%以上。结论酶消化法用于血管平滑肌细胞的培养有利于获得大量的高纯度细胞供实验研究。Objective To culture vascular smooth muscle cells of aged Sprague Dawley（SD） rats using enzymatic dissociation method and provide a large number of primary smooth muscle cells for research of vascular diseases.Methods Aortas of aged SD rats acquired sterilely were subjected to digestion.Vascular smooth muscle cells were digested and dissociated by 0.2% Ⅱ collagenase,purified by natural purification and differential adhesion.α actin of smooth muscle cells were identified by immunohistochemistry.Results Immunohistochemical staining demonstrated the cell purity was more than 95%.Conclusion Enzymatic digestion method employed in vascular smooth muscle cell culture is conducive to obtaining a large number of cells with high purity for experimental research.

关 键 词：细胞培养技术 肌 平滑 血管 免疫组织化学 肌动蛋白类 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]