葡萄内酯在小鼠体内的组织分布  

Tissue distribution of auraptene in mice

作　　者：袁金斌[1] 李明[1] 陈海芳[1] 张瑜[1] 刘莹莹[1] 杨武亮[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《中国新药杂志》2011年第23期2313-2315,共3页Chinese Journal of New Drugs

基　　金：国家"重大新药创制"科技重大专项(2009ZX09103-350);国家自然科学基金(30660230);江西省卫生厅科研课题(2009A057)

摘　　要：目的:考察灌胃给药后葡萄内酯在小鼠体内各个组织中的分布情况。方法:给药后,于不同时间点取心、肝、脾、肺、肾、脑、胃、肠、肌肉、脂肪10个组织,制成组织匀浆,用乙酸乙酯萃取富集,氮吹处理后甲醇定容,采用高效液相色谱法测定并计算各个组织中葡萄内酯的浓度。结果:给药后30 min各组织中药物浓度由高到低依次为:胃>肠>肝脏>脂肪>肾>心脏>肌肉>肺>脾>脑;给药后120 min心、肝、脾、肾、脑等组织中出现分布高峰;480 min后依次消除。结论:葡萄内酯能被迅速吸收并广泛分布到各个组织,但吸收消化的过程比较持久,能透过血脑屏障,在靶器官中的作用时间>8 h。Objective： To determine the distribution of auraptene in mouse tissues after oral adminstration. Methods： After oral administration of auraptene, the heart, liver, spleen, lungs, kidney, brain, stomach, intestine, muscle and fat tissues were isolated and homogenized. The homogenate was extracted with ethyl acetate, and evaporated to dry under nitrogen atmosphere. The contents of auraptene in various samples were determined by HPLC method. Results： At 30 min after administration, the contents of auraptene in tissues were in the following order： stomach 〉 intestine 〉 liver 〉 fat tissues 〉 kidney 〉 heart 〉 muscle 〉 lung 〉 spleen 〉 brain. At 120 min, the peak contents were found in heart, liver, spleen, kidney and brain. At 480 min, the contents of auraptene in all the investigated tissues decreased significantly. Conclusion： The distribution of auraptene in mice is extensive and rapid, but the digestion and absorption are slow and enduring. Auraptene can permeate blood-brain barrier, and the action in target tissues may last for more than 8 h.

关 键 词：葡萄内酯 组织分布 小鼠 高效液相色谱法 

分 类 号：R96[医药卫生—药理学]