盐酸吉西他滨壳聚糖纳米粒的制备及其体外释药特性研究  

Preparation and in vitro release characteristics of gemcitabine hydrochloride chitosan nanoparticles

作　　者：石士慧[1] 潘卫东[2] 

机构地区：[1]江苏省连云港市第二人民医院东院药剂科,连云港222006 [2]江苏联合职业技术学院连云港中医药分院,连云港222006

出　　处：《中国新药杂志》2011年第23期2372-2376,共5页Chinese Journal of New Drugs

摘　　要：目的:优化盐酸吉西他滨壳聚糖纳米粒的制备参数,考察纳米粒体外释药特性。方法:以壳聚糖为辅料,采用离子交联法制备盐酸吉西他滨壳聚糖纳米粒,以包封率、载药量、粒径为参考指标设计试验,确定优化制备参数,以透射电镜观察其表观特征,考察纳米粒体外释药程度。结果:以优化参数制备的盐酸吉西他滨壳聚糖纳米粒包封率为(78.93±1.52)%,载药量为(11.71±0.88)%,纳米粒的平均粒径为(169±24)nm,体外释放试验表明纳米粒中盐酸吉西他滨的释放过程符合Higuchi方程。结论:盐酸吉西他滨可以通过离子交联法制备壳聚糖纳米粒,其粒径、包封率、载药量可控,具有缓释效果。Objective： To optimize the preparing method of gemcitabine hydrochloride chitosan nanoparticles （dFdC-CS-NPs）, and investigate the in vitro release characteristics. Methods： The dFdC-CS-NPs were prepared using ionic gelation method with chitosan. The experiment method was designed based on evaluation of the mean particle size, the encapsulation efficiency and drug loading of dFdC-CS-NPs. The nanoparticles were observed by TEM, and the release curve was determined. Results： The nanoparticles observed by TEM were spherical with a mean particle size of （ 169 ± 24） nm, the encapsulation efficiency and drug loading of dFdC-CS-NPs were （78.93 ± 1.52） % and （ 11.71 ±0.88） %. Release curve of dFdC-CS-NPs fit Higuchi equation. Conclusions： Ionic gelation is applicable to the preparation of dFdC-CS-NPs ; the product has exhibited sustained release in vitro ; and the particle size, EE and CL are controllable.

关 键 词：盐酸吉西他滨 壳聚糖 纳米粒 药物释放 

分 类 号：R943.4[医药卫生—药剂学] R979.1[医药卫生—药学]