大泷六线鱼(Hexagrammos otakii)荧光AFLP分析体系的建立及优化  

Establishment and optimization of fluorescence-based AFLP markers for Hexagrammos otakiis

作　　者：仝颜丽[1] 李海东[2] 王玲[3] 王波[1] 郑明刚[1] 

机构地区：[1]国家海洋局第一海洋研究所,山东青岛266061 [2]日照职业技术学院,山东日照276826 [3]青岛大学,山东青岛266061

出　　处：《海洋环境科学》2011年第6期838-842,共5页Marine Environmental Science

基　　金：国家海洋公益性行业科研专项经费项目(201009);国家农业科技成果转化资金项目(2009-511-208)

摘　　要：以大泷六线鱼为材料,通过对DNA提取质量和浓度、EcoRⅠ/MseⅠ酶切反应时间、Mg2+浓度、dNTPs浓度、引物浓度以及选择性扩增中预扩增产物稀释倍数等关键因素进行研究,建立并优化了大泷六线鱼荧光AFLP分子标记反应体系。用优化的反应体系,筛选到10对适合大泷六线鱼的引物,为今后利用AFLP标记技术进行大泷六线鱼的遗传多样性研究提供了标准化程序。The high quality genomic DNA of Hexagrammos otakii was extracted and the optimized fluorescence-based AFLP（amplified fragment length polymorphism） analysis system was established for H.otakii.In this study,the main factors influencing the results of AFLP were optimized,including the quality and concentration of the extracted DNA,reaction time of enzymes EcoRl/MseI digestion,and the concentration of Mg2＋,DNTPs.The selective primer and the dilute multiple of pre-amplification production were also optimized in selective amplification reaction system.Ten pairs of primers were identified from eighty-one pairs of primers by optimizing the AFLP reaction system.The result showed a strong foundation for the AFLP analysis of H.otakii,which provide a standardized program for studying the genetic diversity of H.Otakii.

关 键 词：大泷六线鱼 荧光AFLP DNA提取 

分 类 号：Q331[生物学—遗传学]