肝再生磷酸酶-3小干扰RNA对异位子宫内膜间质细胞骨架的影响  

The effects of PRL-3 siRNA on the endometriotic stromal cells cytoskeleton

作　　者：阮菲[1] 吴瑞瑾[1] 徐开红[1] 涂飞霞[1] 鲁东红[1] 马俊彦[1] 费向炜[1] 陈利青[1] 梁峥[1] 林俊[1] 

机构地区：[1]浙江大学医学院附属妇产科医院,浙江杭州310006

出　　处：《中国实用妇科与产科杂志》2011年第12期925-927,共3页Chinese Journal of Practical Gynecology and Obstetrics

基　　金：2009年高等学校博士学科点专项科研基金(No.20090101110123)

摘　　要：目的探讨肝再生磷酸酶-3小干扰RNA(PRL-3 si RNA)对异位子宫内膜间质细胞骨架的影响。方法选择2009年11月至2010年5月在浙江大学医学院附属妇产科医院行卵巢内异囊肿剔除术的子宫内膜异位症患者32例,原代培养异位子宫内膜间质细胞并鉴定,运用siRNA技术干扰沉默PRL-3基因,Westernblot检测PRL-3蛋白表达,激光扫描共聚焦显微镜观察PRL-3 si RNA对异位子宫内膜间质细胞骨架的影响。结果经PRL-3 si RNA干扰后PRL-3蛋白表达明显下降,与对照组相比较,实验组异位子宫内膜间质细胞中应力纤维数目明显增多、变粗而清晰,而细胞的丝状、板状伪足却明显减少。结论 PRL-3 si RNA能有效沉默PRL-3,并且使异位子宫内膜间质细胞骨架构型显著改变,从而导致异位子宫内膜细胞迁移能力下降。Objective To evaluate the effects of PRL-3 siRNA on the endometriotic stromal cells cytoskeleton by laser scanning confocal microscopy. Methods Endometriotic stromal cells were primarily cuhured and identified in vitro. SiRNA was designed to silence PRL-3 gene, and PRL-3 protein expression was detected by western blot. Then endometriotic stromal cells cytoskeleton was observed by laser scanning confocal microscopy. Results The expression of PRL-3 protein significantly decreased in experimental group, compared with control group. Furthermore, stress fibers became thick and clear in the experimental group, while filaments and pseudopodia plate significantly reduced Compared with control group. Conclusion PRL-3 siRNA could silence PRL-3 gene, make significant changes of endometriotic stromal cells cytoskeleton distribution, which decreased cell migration ability. It suggested that PRL-3 may be a potentially attractive therapeutic strategy in endometriosis.

关 键 词：子宫内膜异位症 肝再生磷酸酶-3 小干扰RNA 激光扫描共聚焦显微镜 细胞骨架 

分 类 号：R711.71[医药卫生—妇产科学]