复方苦参注射液含量测定方法研究  

Determination of matrine and glucose injection by HPLC

作　　者：刘军玲[1] 

机构地区：[1]安徽省食品药品检验所,安徽合肥230051

出　　处：《安徽医药》2011年第12期1516-1517,共2页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立HPLC法测定复方苦参碱注射液中苦参碱与氧化苦参碱的总量。方法采用氨基柱(250 mm×4.6 mm,5μm),以乙腈-无水乙醇-3%磷酸溶液(80∶10∶10)为流动相,检测波长为220 nm,HPLC法测定复方苦参注射液中苦参碱与氧化苦参碱的总量。结果 HPLC测得苦参碱进样量在0.195 66～2.348 16μg范围内线性关系良好(r=0.999 4),氧化苦参碱进样量在0.375 84～4.510 08μg范围内线性关系良好(r=0.999 5),平均回收率为97.3%,RSD为1.0%。结论该法准确,重复性好,可用于控制复方苦参碱注射液的质量。Aim To estalish an HPLC method for the determination of matrine and glucose injection.Methods Matrine can be separated on NH2 column（NH2 250 mm×4.6 mm,5 μm）with a mixture of acetonitrile dehydrated ethanol and 3%phosphoric（80∶ 10∶ 10） as mobile phase and detected at 220 nm.The flow rate was 1.0 ml·min^-1.Results Matrine showed a good linear relationship at a range of 0.195 66-2.348 16 μg（r=0.999 4）.Oxyatrine showed a good linear relationship at a range of 0.375 84~4.510 08 μg（r=0.999 5）.The average recovery was 97.3%,and RSD was 1.0%.Conclusion The method is accurate and has a good reproducibility.It can be used for the quality control of matrine and glucose injection.

关 键 词：高效液相色谱法 苦参碱 氧化苦参碱 

分 类 号：R286.0[医药卫生—中药学]