家蚕滞育卵与非滞育卵中几种关键酶活性的比较  

Comparison of related enzyme activities between diapause and non-diapause eggs of the silkworm,Bombyx mori

作　　者：范兰芬[1] 钟杨生[1] 林健荣[1] 

机构地区：[1]华南农业大学动物科学学院,广州510642

出　　处：《昆虫学报》2011年第11期1258-1263,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(30771634);现代农业产业技术体系建设专项资金(蚕桑)(CARS-22)

摘　　要：家蚕Bombyxmori是卵滞育的昆虫,在滞育期间无形态变化,也不存在器官发育和组织分化,然而其生理代谢过程仍在进行。为进一步研究家蚕滞育的机制,本研究测定了家蚕滞育卵、即时浸酸处理的滞育卵及非滞育卵在胚胎发育过程中的超氧化物歧化酶(superoxide dismutase,SOD,EC1.15.1.1)、过氧化氢酶(catalase,CAT,EC1.11.1.6)、丙酮酸激酶(pyruvate kinase,PK,EC2.7.1.40)、乙酰胆碱酯酶(acetylcholine esterase,AchE,EC3.1.1.7)和乳酸脱氢酶(lactate dehydrogenase,LDH,EC1.1.1.28)的活性变化。结果表明:处理后1-7d,即时浸酸处理的滞育卵,SOD活性由56517.00U/g提高到81986.94U/g,CAT活性由14.98U/g提高到106.90U/g,PK活性由25.19U/g提高到181.70U/g,AChE活性由17.88U/g提高到287.86U/g,而LDH活性由169.96U/g下降到122.82U/g。而在非滞育卵中,SOD活性由86417.99U/g下降到66024.19U/g,LDH活性由169.07U/g下降到135.02U/g;CAT活性由1.47U/g提高到44.37U/g,PK活性由20.56U/g提高到92.09U/g,AChE活性由21.40U/g提高到99.17U/g。在滞育卵中,SOD和AChE活性较稳定;CAT活性随发育上升,而LDH活性随发育而下降;PK活性在胚胎发育的前4d呈上升趋势,随后基本保持稳定。通过了解家蚕滞育卵、非滞育卵与即时浸酸卵的相关酶活性在胚胎发育过程中存在的变化,有助于进一步揭示家蚕滞育的机理。The silkworm Bombyx mori is an egg-diapause insect.There are no morphological changes,organ development and tissue differentiation during diapause stage.However,its physiological metabolism is still in progress.In order to further research the diapause mechanism,the change in activities of superoxide dismutase（SOD,EC 1.15.1.1）,catalase（CAT,EC 1.11.1.6）,pyruvate kinase（PK,EC 2.7.1.40）,acetylcholine esterase（AchE,EC 3.1.1.7） and lactate dehydrogenase（LDH,EC 1.1.1.28） in the early embryonic stage of diapause eggs,diapause eggs with instant hydrochloric acid（HCl） soaking and non-diapause eggs were detected in this study.The results showed that the activities of SOD,CAT,PK and AChE in the HCl-soaked eggs,from 1 d to 7 d,increased from 56 517.00 U/g to 81 986.94 U/g,14.98 U/g to 106.90 U/g,25.19 U/g to 181.70 U/g,and 17.88 U/g to 287.86 U/g,respectively,while that of LDH decreased from 169.96 U/g to 122.82 U/g.In the non-diapause eggs during the same period,the activities of SOD and LDH decreased from 86 417.99 U/g to 66 024.19 U/g and 169.07 U/g to 135.02 U/g,respectively;the activities of CAT,PK and AchE,however,increased from 1.47 U/g to 44.37 U/g,20.56 U/g to 92.09 U/g,and 21.40 U/g to 99.17 U/g,respectively.In the diapause eggs,the activities of SOD and AChE were stable;the CAT activity was raised but LDH activity was decreased with the embryonic development;and the PK activity was raised in the first four days of the embryonic development and remained stable afterwards.Understanding the change of related enzyme activities among diapause,non-diapause and HCl-soaked silkworm eggs in the embryonic development will help to reveal the diapause mechanism.

关 键 词：家蚕 滞育 滞育卵 非滞育卵 即时浸酸处理 酶活性 

分 类 号：Q966[生物学—昆虫学]