黄荆子乙酸乙酯提取物及木脂类化合物对人卵巢癌COC1细胞体外增殖及裸鼠皮下移植瘤的抑制作用  

The acetoacetate extract of vitex negundo seed and lignans inhibit the growth of ovarian carcinoma cells both in vitro and in vivo

作　　者：邓宇傲 谢宛玉[2] 李秀云 曹建国[3] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院妇科,深圳518020 [2]南华大学附属第一医院妇产科 [3]湖南师范大学药学工程实验室

出　　处：《现代妇产科进展》2011年第11期863-867,共5页Progress in Obstetrics and Gynecology

摘　　要：目的:研究黄荆子乙酸乙酯提取物EVn-50及从中分级分离获得的4种木脂类化合物(Comp 6、Comp 7、Comp 8、Comp 10)体内外对人卵巢癌COC1细胞的作用。方法:体外培养人卵巢癌COC1细胞,细胞计数法检测EVn-50、Comp 6、Comp 7、Comp 8、Comp 10对人卵巢癌COC1细胞生长的影响;采用人卵巢癌COC1细胞裸鼠移植瘤模型治疗实验评价EVn-50、Comp 6、Comp 7、Comp 8、Comp 10体内治疗卵巢癌的有效性。结果:EVn-50、Comp 6、Comp 7、Comp 8、Comp 10对人卵巢癌COC1细胞生长具有抑制作用,且呈浓度及时间依赖性,其中,以Comp 8效价最高,其IC50为1.40μg/ml;其次为EVn-50,其IC50为1.56μg/ml;体内实验显示,5mg/kg给药20天时,EVn-50、Comp 6对人卵巢癌COC1细胞裸鼠皮下移植瘤生长抑制率分别为57.1%、55.7%;瘤重抑制率分别是61.6%、53.1%,Comp 8无效。结论:黄荆子乙酸乙酯提取物EVn-50及黄荆子木脂类化合物体内外均能抑制人卵巢癌COC1细胞的生长和增殖。Objective：To investigate the therapeutic effect of the acetoacetate extract of Vitex Negundo Seed（EVn-50）and four fractions of Lignans from EVn-50（Comp 6,Comp 7,Comp 8,and Comp 10）on human ovarian cancer COC1 cell line.Methods：COC1 cells that cultured in vitro were divided into five experimental groups（EG）and three control groups（CG）.Cells were treated with different concentrations of EVn-50,Comp 6,Comp 7,Comp 8,Comp 10 and control drug.The trypan blue exclusion method was used to investigate the growth inhibitory effect of EVn-50 and four fractopms of Lignans of Vitex negundo seed on COC1 cells.The established tumor xenograft in nude mouse model was used to evaluate therapeutic effect of EVn-50 and four fractions of Lignans on human ovarian cancer.Results：EVn-50 and Comp 6,Comp 7,Comp 8,Comp 10 all inhibited viability of COC1 cells significantly in a dose-dependent manner and a time-dependent manner in vitro.Comp 8 was the strongest one with IC50 value of 1.40μg/ml.EVn-50 was the second one with IC50 of 1.56 μg/ml.The tumor growth inhibitory effect of EVn-50 and Comp 6 at a dose of 5mg/kg were 57.1% and 55.7%respectively.The tumor weight inhibitory rates were 61.6%and 53.1% respectively.Comp 8 had little effect.Conclusion：The acetoacetate extract of Vitex negundo seed（EVn-50）and the Lignans from the acetoacetate extract of Vitex negundo seed have an inhibiting effect on the growth of human ovarian cancer COC1 cell line both in vitro and in vivo.

关 键 词：卵巢癌 黄荆子 黄荆子乙酸乙酯提取物 木脂素 治疗作用 

分 类 号：R445[医药卫生—影像医学与核医学]