原花青素对STZ致胰岛细胞损伤的保护作用  

The protection effects of proanthocyanidins on STZ-injured INS-1 cells

作　　者：赵丽[1] 陈红梅[2] 蔺美玲[2] 刘世雄[3] 李中平[1] 王志强[1] 张小郁[2] 郑天珍[2] 

机构地区：[1]兰州大学基础医学院生理学与心理学研究所,甘肃兰州730000 [2]甘肃省新药临床前研究重点实验室 [3]兰州大学第一医院老年科

出　　处：《中国老年学杂志》2011年第23期4580-4582,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金资助项目(No.30572428)

摘　　要：目的探讨原花青素(PC)对链脲佐菌素(STZ)损伤大鼠胰岛素瘤细胞(INS-1)的保护作用。方法 INS-1细胞培养24 h后,以链脲佐菌素(STZ)损伤细胞,将细胞分为正常对照组、STZ组、PC保护组(PC+STZ),PC保护组培养液中加入不同浓度的PC干预12 h后,用MTT比色法测定细胞存活率,用放免法检测培养细胞上清液胰岛素分泌量的变化,用比色法测定培养细胞上清液丙二醛(MDA)和超氧化物歧化酶(SOD)等指标的变化。结果 PC可以减少STZ对INS-1细胞的损伤,PC保护组的INS-1细胞存活率增加(P<0.01);与STZ组比较,PC保护组促进了INS-1细胞的胰岛素分泌(P<0.01),降低了INS-1细胞的MDA含量(P<0.01),增强了T-AOC的活性(P<0.01)。结论原花青素对STZ损伤INS-1细胞有一定的保护作用,其机制可能是通过降低MDA生成,提高T-AOC能力有关。Objective To investigate the protective effects of proanthocyanidins on STZ-injured INS-1 cells.Methods INS-1 cells were seeded into culture plates,and incubated for 24 h,they were divided into normal control,STZ,protection groups（proanthocyanidins ＋STZ）.Graded concentrations of proanthocyanidins were added into the culture medium of STZ injured INS-1 cells in protection group,respectively.The survival rate was measured by MTT assay.Insulin secretion was measured by radio-immunoassay.Supernatant malondialdehyde（MDA） and total anti-oxidation capability（T-AOC） were determined by colorimetric assay.Results Compared with that of STZ group,OD value was significantly increased in proanthocyanidins＇s protection（P〈0.01）.In above two groups,insulin secretion was significantly increased（P〈0.01）.MDA production was markedly reduced,and T-AOC activity was evaluated in above two groups（P〈0.01）.Conclusions Proanthocyanidins has protection effects on STZ-injured INS-1 cells,which its mechanisms may be partly via MDA reduction,T-AOC elevation.

关 键 词：原花青素 链脲佐菌素 INS-1细胞 胰岛素 

分 类 号：R965.1[医药卫生—药理学]