载脂蛋白E在骨骼肌系统的表达研究  

Gene Expression of Apolipoproteins E in Skeletal Muscle

作　　者：张慧[1] 范乐明[1] 蔡海江[1] 

机构地区：[1]南京医科大学,动脉粥样硬化研究中心210029

出　　处：《心肺血管病杂志》1999年第4期284-287,共4页Journal of Cardiovascular and Pulmonary Diseases

基　　金：江苏省教委自然科学基金

摘　　要：本研究利用骨骼肌合成和分泌非肌性蛋白质，进而分泌入血并在细胞外发挥作用的原理，构建了一个由人巨细胞病毒早期增强子和促进子驱动的人ａｐｏＥｃＤＮＡ 表达质粒（ｐＣＭＶａｐｏＥ） ，通过阳离子脂质体介导转染原代培养的小鼠骨骼肌肌母细胞，ＥＬＩＳＡ和免疫组织化学检测均证实ａｐｏＥ 在肌源性细胞成功表达并分泌至培养液中。直接肌肉注射，ｐＣＭＶａｐｏＥ 表达载体也在小鼠骨骼肌中成功表达，并且预先经氯化钡诱导损伤可使其表达量明显增加。Non-muscular proteins can be expressed in it and non-muscular proteins could be secreted to the circulation.We constracted a plasmid vector containing the human apoE cDNA,driven by human cytomegalovirus major immediate early enhancer/promoter(pCMV apoE).Primary cultured myoblasts were transfected with pCMV apoE mediated by lipofectamin.The result of both ELISA and immunocytochemistry showed that apoE expressed in the myocytes and secreted into the culture medium;pCMV apoE expressed in the skeletal muscle by injection and the expression was increased singnificantly if pre-treated with barium chloride.These results provided some exprimental accordings for atherosclerosis gene therapy.

关 键 词：载脂蛋白E 骨骼肌 基因表达 药理 

分 类 号：R977.6[医药卫生—药品]