高效液相色谱法测定人血清中头孢曲松浓度  

Determination of Ceftriaxone in Human Serum by HPLC

作　　者：苗奕[1] 刘真业[1] 季小慎[1] 张华峰[1] 

机构地区：[1]空军总医院药理科,北京100036

出　　处：《药物分析杂志》1999年第6期382-384,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的： 建立一种高效液相色谱快速测定人血清中头孢曲松浓度的分析方法。方法： 使用Ｂｉｏ－ Ｒａｄ 高效液相色谱仪， ＬｕｎａＴＭ Ｃ１８ （２） 柱（４－６ ｍ ｍ ×１５０ ｍ ｍ ， ５ μｍ） ， 流动相为甲醇－ ０－１ ％ 三乙胺水溶液（１５∶８５ ， 用磷酸调ｐＨ 至７－５） ， 检测波长为２４０ ｎｍ ， 内标为茶碱。结果： 头孢曲松人血清浓度在０－７５ ～３８４ μｇ·ｍ Ｌ－ １ 范围内线性良好， 最低检出浓度为０－５ μｇ·ｍ Ｌ－ １ ， 日内及日间精密度均优于５ ％ 。结论： 本方法经济、快速、灵敏、准确、专属性高。Objective:To establish a simple HPLC method for the determination of ceftriaxone in human serum Method: A Bio-Rad 1350t HPLC instrument with Luna TM C 18 (2) (4 6 mm×150 mm,5 μm) column was used The mobile phase was methanol-0 1% triethyl amine aqueous solution (15∶85, pH was adjusted to 7 5 by phosphoric acid).UV detector was set at 240 nm and theophylline was used as internal standard Results: A linear relationship was obtained between the peak height ratio and the concentration of ceftriaxone from 0 75 to 384 μg·mL -1 and the limit of detection of ceftriaxone in serum was 0 5 μg·mL -1 Both intraday and interday precisions were better than 5% Conclusion: The method is economic, rapid, sensitive and accurate

关 键 词：头孢曲松 高效液相色谱法 血清 药代动力学 

分 类 号：R978.11[医药卫生—药品] R969.1[医药卫生—药学]