脑缺血大鼠凝血酶原和PAR-1的变化及滋阴活血解毒方的干预作用  

Study on changes of Prothrombin mRNA and PAR-1 mRNA expression in focal cerebral infarction in rats and intervening effects of Ziyin Huoxue Jiedu decoction

作　　者：邓奕辉[1] 陈懿[1] 邓婧溪[1] 段春艳[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208

出　　处：《湖南中医药大学学报》2011年第11期16-18,共3页Journal of Hunan University of Chinese Medicine

基　　金：湖南省自然科学基金资助项目(10JJ6047);湖南省教育厅青年基金资助项目(08B005);国家中医药管理局中西医结合临床重点学科资助

摘　　要：目的观察局灶性脑缺血模型大鼠脑组织凝血酶原(prothrombin,F2)mRNA和蛋白酶激活受体-1(pro-tease-activated receptor-1,PAR-1)mRNA表达变化及滋阴活血解毒方的干预作用。方法将24只大鼠随机分为假手术组、模型组、滋阴活血解毒方组、阿加曲班4组,每组分1、3 d两个时相点。制备大脑中动脉闭塞(MCAO)模型,检测各组大鼠脑组织中凝血酶原mRNA及PAR-1mRNA的表达水平。结果与假手术组比较,模型组脑组织中凝血酶原mRNA、PAR-1mRNA表达明显增强(P<0.01),滋阴活血解毒方组和阿加曲班组能明显下调凝血酶原mRNA、PAR-1mRNA表达(P<0.01)。结论局灶性脑缺血大鼠凝血酶原mRNA和PAR-1mRNA的表达增强,滋阴活血解毒方能抑制其表达,减轻凝血酶的毒性反应。Objective To observe the changes of Prothrombin mRNA and PAR-1 mRNA expression in focal cerebral infarction in rats and intervening effects of Ziyin Huoxue Jiedu decoction.Methods Twenty-four rats were randomly divided into 4 groups： the sham-operated,model,Ziyin Huoxue Jiedu decoction and Argatroban groups.Each group was divided into two time phase point of 1st day and 3rd day.The MCAO models were reproduced.The expression of prothrombin mRNA and PAR-1 mRNA in brain tissue were detected.Results Compared with the sham-operated group,the expression of prothrombin mRNA and PAR-1 mRNA in brain tissue in model group were significantly higher（P0.01）,the expression of prothrombin mRNA and PAR-1 mRNA were lowered significantly in Ziyin Huoxue Jiedu decoction group and Argatroban Injection group（P0.01）.Conclusion Ziyin Huoxue Jiedu decoction is effective to restrain the expression of prothrombin mRNA and PAR-1 mRNA in brain tissue and reduce thrombin toxic injury.

关 键 词：局灶性脑缺血 凝血酶原mRNA PAR-1mRNA 大鼠 滋阴活血解毒方 熟地 川芎 黄芩 

分 类 号：R285.5[医药卫生—中药学]