复方三七片质量标准研究  

Studies on quality standard of compound pseudo-Ginseng tablets

作　　者：李卓[1] 邓桂明[1,2] 郭昱[1] 王文娜[1] 张鹤鸣[1] 

机构地区：[1]华南师范大学中药及创新药物研究所国家中医药管理局中医药与光子技术三级实验室,广东广州5106312 [2]湖南中医药大学第一附属医院药剂科,湖南长沙410007

出　　处：《湖南中医药大学学报》2011年第11期34-37,共4页Journal of Hunan University of Chinese Medicine

摘　　要：目的研究成方制剂复方三七片的质量标准。方法采用TLC对白芷、三七进行定性鉴别;采用HPLC进行主成分含量测定,色谱柱为Aglient ZORBAX C18柱(150 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱,流速为1.0 mL/min,检测波长为203 nm。结果 TLC可鉴别三七和白芷,HPLC测定人参皂苷Rg1、人参皂苷Rb1和三七皂苷R1,三者分别在0.048～0.384 mg、0.052 5～0.420 mg、0.011 8～0.094 4 mg范围内线性、精密度、稳定性均良好,阴性无干扰,平均加样回收率分别为100.91%(RSD=1.04%)、100.36%(RSD=1.04%)、100.29%(RSD=0.99%)。结论所建立的质量标准简便可行、重复性好,可以用来评价复方三七片的质量。Objective To establish a quality standard for compound Pseudo-Ginseng tablets Methods Angelica dahurica and Pseudo-Ginseng were identified by thin layer chromatography.The content of Ginsenoside Rg1,Ginsenoside Rb1 and Notoginsenoside R1 were determined by High Performance Liquid Chromatography.Chromatographic conditions： Aglient ZORBAX C18 column（150 mm×4.6 mm,5 μm） was used as the stationary phase,a mixture of acetonitrile and water as the mobile phase was adopted.The flow rate was 1.0 mL/min,and the detection wavelength at 203 nm.Results The qualitative identification with TLC were specific.The linear response range was 0.048～0.384 mg/mL for Ginsenoside Rg1,0.0525～0.420 mg/mL for Ginsenoside Rb1,and 0.0118～0.0944 mg/mL for Notoginsenoside R1.The average recoveries of these compounds were 100.91%（RSD=1.04%）,100.36%（RSD=1.04%）,100.29%（RSD=0.99%）,respectively.Conclusion This method is simple,accurate and reproducible for quality control of Compound Pseudo-Ginseng Tablet.

关 键 词：复方三七片 质量标准 HPLC TLC 人参皂苷RG1 人参皂苷RB1 三七皂苷R1 

分 类 号：R284.1[医药卫生—中药学]