糖尿病大鼠糖化终产物与主动脉壁细胞间粘附分子-1关系的研究  

The relationship between advanced glycosylation end products and expression of intercellular adhesion molecule 1 of aorta in diabetic rats

作　　者：余路[1] 邱鸿鑫[1] 王亚平[1] 吴珊[1] 司良毅[1] 李昕[1] 李媛媛[1] 姜蓉[1] 祝继华[1] 粟绍初[1] 李萍[1] 

机构地区：[1]重庆医科大学临床医学院内分泌科

出　　处：《中华内分泌代谢杂志》1999年第6期346-349,共4页Chinese Journal of Endocrinology and Metabolism

摘　　要：目的　了解糖尿病大鼠体内糖化终产物（ＡＧＥｓ） 的产生与主动脉壁细胞间粘附分子１（ＩＣＡＭ１） 表达的关系， 并在体外确定ＡＧＥｓ 是否具有独立致内皮细胞ＩＣＡＭ１ 表达的作用。方法将糖尿病大鼠（ＤＭ 组） 、糖尿病氨基胍治疗大鼠（ＡＧ 组） 和正常大鼠（Ｃ 组） 分别喂养１、２、３ 、４ 个月后，测定其ＨｂＡＧＥｓ 含量及主动脉壁ＩＣＡＭ１ 表达情况。用流式细胞仪和半定量ＥＬＩＳＡ检测培养的内皮细胞在ＡＧＥｓ 作用后ＩＣＡＭ１ 生成量的变化。结果　ＤＭ 组各时相点ＨｂＡＧＥｓ 逐月增高〔１ ～４个月分别为（６．８８±１．２３） 、（１０ ．２６ ±０ ．６３）、（１５ ．３０ ±１ ．４９） 、（１８．５７±２．９０）ＡＧＥｓ Ｕ／ｍｇ〕，均显著高于ＡＧ组和Ｃ组（Ｐ＜０ ．０５），其增高主要与高血糖相关。ＤＭ 组内皮ＩＣＡＭ１ 免疫组织化学染色阳性率显著高于ＡＧ组及Ｃ组，１ ～４ 个月分别为１７％ 、７１ ％ 、７５ ％ 、７１％ （Ｐ＜ ０．０５） 。流式细胞仪测定的内皮细胞在ＡＧＥｓ 刺激４ 、６ 小时后ＩＣＡＭ１ 表达阳性的细胞分别占（４．１１±０．３６） ％ 、（１６ ．１１ ±１ ．０７）％ ，较正常对照细胞明显升高。半定量ＥＬ?Objective To explore the relationship between advanced glycosylation end products (AGEs) and expression of intercellular adhesion molecule 1 (ICAM 1) of aorta in diabetic rats and to investigate whether the ICAM 1 expression of cultured endothelial cells (ECs) can be caused by AGEs independently. Methods Wistar rats were divided into three groups: STZ induced diabetic rats (group DM), aminoguanidine (AG) treated diabetic rats (group AG) and the controls (group C). After 1,2,3 and 4 months, the level of Hb AGEs was assayed and ICAM 1 expression of aorta was determined by immunohistochemistry. The production of ICAM 1 of cultured ECs after AGEs stimulation was detected by both flowcytometry (FCM) and semi quantitative ELISA. Results Hb AGEs in DM group was elevated significantly as compared with group AG and group C, increased with the duration of the disease 〔after 1,2,3 and 4 months the Hb AGEs being (6.88±1.23), (10.26±0.63), (15.30±1.49) and (18.57±2.90) AGEs U/mg, respectively〕, and closely related to the increasing levels of fasting blood glucose. The positive rates of ICAM 1 staining in group DM (being 17%, 71%, 75% and 71% respectively after 1,2,3 and 4 months) were significantly higher than those of group AG and group C (P<0.05). After 4h and 6h stimulation with AGEs, the counts of ICAM 1 expression by ECs were (4.11±0.36)% and (16.11±1.07)% as detected by FCM, and the absorbance of semi quantitativeELISAwas0.24±0.03and0.65±0.14, while the adhesion rates were (32.89±2.65)% and (54.55±5.62)% respectively, all significantly higher than those of untreated ECs. Conclusion AGEs may be an important factor for the expression of ICAM 1 of aorta both in vivo and in vitro.

关 键 词：糖化终产物 糖尿病 内皮细胞 细胞间附分子 

分 类 号：R587.102[医药卫生—内分泌]