超声靶向微泡破碎联合半乳糖聚乙烯亚胺介导EGFP质粒转染肝癌细胞的研究  

Efficiency of EGFP gene transfection in HepG2 cells mediated by ultrasound-targeted microbubble destruction and PEI-Gal

作　　者：张园[1] 李银鹏[1] 吕锋[1] 王娜[1] 张海[1] 朱惠明[1] 

机构地区：[1]暨南大学附属第二临床医学院消化病科,深圳市518020

出　　处：《江苏医药》2011年第23期2751-2754,F0002,共5页Jiangsu Medical Journal

基　　金：深圳科技计划项目(201002015)

摘　　要：目的探讨超声靶向微泡破碎(UTMD)联合半乳糖聚乙烯亚胺(PEI-Gal)介导pEGFP-N3质粒转染肝癌细胞HepG2的效率及对细胞增殖的影响。方法体外培养HepG2细胞,随机分为6组:(1)空白对照组(C);(2)超声(U)+微泡(M)组;(3)PEI-Gal(P)组;(4)P+M组;(5)P+U组;(6)P+U+M组。转染24h后荧光显微镜观察HepG2细胞中绿色荧光蛋白质的表达,流式细胞术检测转染率,MTT法检测细胞活性。结果 P+U+M组转染率为(29.16±1.78)%,明显高于其他各组(P均<0.05),U+M组转染率最低(15.63±1.81)%。P+U+M组存活率为(83.48±0.93)%,低于U+M组(86.37±1.56)%和P+U组的(89.54±0.91)%(P<0.01)。结论 UTMD联合PEI-Gal能显著增强pEGFP-N3质粒转染肝癌细胞HepG2的效率。Objective To investigate the transfection efficiency and safty of plasmid pEGFP-N3 transfection into HepG2 cells mediated by ultrasound-targeted microbubble destruction（UTMD） and PEI-Gal.Methods The HepG2 cells were cultured in 24-well dishes and divided into 6 groups of blank control（C）,ultrasound and microbubble group（U＋M）,PEI-Gal group（P）,PEI-Gal and ultrasound group（P＋U）,PEI-Gal and microbubbles（P＋M）,PEI-Gal plus ultrasound and microbubbles group（P＋U＋M）.At 24 hours after transfection,the EGFP gene expression in HepG2 cells was detected by fluorescence microscopy and flow cytometry.The survival rate of cells was measured by MTT.Results Compared with the other groups,transfection efficiency was significantly increased by P＋U＋M（29.16±1.78）%（P0.05）.The survival rate in P＋U＋M group was（83.48±0.93）%,which was lower than（86.37±1.56）% in U＋M group and（89.54±0.91）% in P＋U group（P0.01）.Conclusion UTMD and PEI-Gal can enhance the efficiency of EGFP gene transfection in HepG2 cells.

关 键 词：超声靶向微泡破碎 半乳糖聚乙烯亚胺 基因转染 肝癌 

分 类 号：R318[医药卫生—生物医学工程]