脑创伤程度对大鼠伤后胚胎神经干细胞移植的影响  

Impact of injury severity on transplantation of embryonic neural stem cells following traumatic brain injury in rats

作　　者：洪军[1] 崔建忠[1] 黎洁[2] 刘兴宇[1] 周云涛[2] 

机构地区：[1]河北医科大学附属唐山临床医学院,唐山工人医院神经外科,唐山063000 [2]河北医科大学附属唐山临床医学院中心实验室,唐山063000

出　　处：《中华创伤杂志》2011年第12期1145-1149,共5页Chinese Journal of Trauma

基　　金：河北省科委指令基金资助项目（No.07276101D-49）

摘　　要：目的探讨不同程度创伤性脑损伤（traumatic brain injury，TBI）对伤后胚胎神经干细胞（neural stem cells，NSCs）移植的影响。方法从孕12～14d胚胎大鼠海马组织中分离NSCs，采用无血清培养法，进行体外培养、扩增和鉴定。大鼠分别于轻型、中型TBI后3d行胚胎NSCs双侧海马区移植；细胞移植14d后行组织学和TUNEL检测，并对BrdU、NSE、GFAP、GalC、NGF、BDNF蛋白行免疫组化检测。结果移植治疗后14d，轻型TBI组双侧海马区Brdu阳性细胞数明显多于中型TBI组。移植胚胎NSCs脑内分化以GFAP阳性胶质细胞为主。轻、中型TBI后NGF和BDNF蛋白阳性表达增加，其中以轻型TBI组表达最为显著。结论轻型和中型TBI对NSCs移植的影响与伤后脑组织局部微环境因素的改变密切相关。Objective To explore the influence of injury severity on transplantation of embryonic neural stem cells （NSCs） after traumatic brain injury （TBI）. Methods The NSCs were isolated from the hippocampus of fetal rats aged at from 12-14 days. The cells were cultured and proliferated in the serum-free medium and identified in vitro. The animals received transplants in the bilateral hippocampal areas at day 3 following mild or moderate TBI separately. Conventional histology, TUNEL and immunohistology were examined to detect BrdU, NSE, GFAP, GalC, NGF and BDNF at day 14 post-implantation. Results BrdU-labeled positive cells in the bilateral hippocampus in the mild TBI group were more than those in the moderate TBI group at day 14 post-implantation. Significant differentiation of the astrocytes recognized as GFAP positive cells in the bilateral hippocampus was found at day 14 post-implantation. The expression of NGF and BDNF proteins was increased following TBI, the most evident in the mild TBI group. Conclusion The influence of injury severity on transplantation may be associated with the change of the microenvironment after TBI.

关 键 词：脑损伤 干细胞 胚胎 大鼠 

分 类 号：R651.1[医药卫生—外科学]