绵马贯众的活性成分研究  

Active constituents of dryopteris crassirhizoma Nakai

作　　者：刘钊[1] 安熙强 斯建勇[1] 曹丽[1] 沈连钢[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]新疆维吾尔自治区药物研究所食品检测研究室,新疆乌鲁木齐830004

出　　处：《西部医学》2011年第12期2300-2302,2306,共4页Medical Journal of West China

基　　金：国家高技术研究发展计划(863计划)项目子课题(编号2006AA10A208-3-2);国家重大传染病防治专项2008zx10003-006

摘　　要：目的研究绵马贯众的间苯三酚类活性成分,以确定其抗病毒的物质基础。方法在文献报道的缓冲硅胶柱层析法的基础上,对影响分离效果的三个主要因素进行反复量化试验,以探索快速分离、纯化间苯三酚类化合物的优化方法。结果应用此优化方法,从绵马贯众中分离得到5个化合物,其中4个为间苯三酚类化合物,用波谱学方法分别鉴定为绵马酸ABA(1)、绵马酸ABB(2)、绵马酸ABP(3)、东北贯众素ABBA(4)和二十五烷醇(5),其中(3)为从绵马贯众中首次分离得到的单体化合物。结论优化后的缓冲硅胶柱层析法分离效果好、样品回收率高,适用于绵马贯众的间苯三酚类活性成分研究。Objective To study the phloroglucinol derivatives of dryopteris crassirhizoma Nakai,and determine them anti-virus material basis.Methods The quantitative tests on three main factors which affect separation on the basis of buffered-silica-gel-column-chromatography method were applied to find a rapid method for separation and purification of the phloroglucinol derivatives at last.Results 5compounds including 4phloroglucinol derivatives were separated from the rhizome of Dryopteris Crassirhizoma.On the basis of NMR and MS spectroscopic analyses,these compounds were identified as filixic acid ABA（1）,filixic acid ABB（2）,filixic acid ABP（3）,dryocrassin ABBA（4）and pentacosanol（5）.Compounds（3）were isolated from Dryopteris crassirhizoma Nakai for the first time.Conclusion The optimized buffered-silica-gel-column-chromatography method has a good separation effect and a high sample recovery,which is suitable to study the phloroglucinol derivatives of Dryopteris crassirhizoma Nakai.

关 键 词：绵马贯众 抗病毒 间苯三酚 绵马酸ABP 

分 类 号：TQ050.425[化学工程]