Product Variability of the ‘Cineole Cassette＇ Monoterpene Synthases of Related Nicotiana Species  

作　　者：Anke Fahnrich Katrin Krause Birgit Piechulla 

机构地区：[1]University of Rostock, Institute for Biological Sciences, Albert-Einstein-Str. 3, 18059 Rostock, Germany

出　　处：《Molecular Plant》2011年第6期965-984,共20页分子植物（英文版）

摘　　要：Nicotiana species of the section Alatae characteristically emit the floral scent compounds of the ＇cineole cassette＇ comprising 1,8-cineole, limonene, myrcene, α-pinene, β-pinene, sabinene, and α-terpineol. We successfully isolated genes of Nicotiana alata and Nicotiana langsdorfii that encoded enzymes, which produced the characteristic monoter- penes of this ＇cineole cassette＇ with α-terpineol being most abundant in the volatile spectra. The amino acid sequences of both terpineol synthases were 99% identical. The enzymes cluster in a monophyletic branch together with the closely related cineole synthase of Nicotiana suaveolens and monoterpene synthase 1 of Solanum lycopersicum. The cyclization reactions （α-terpineol to 1,8-cineole） of the terpineol synthases of N. alata and N. langsdorfii were less efficient compared to the ＇cineole cassette＇ monoterpene synthases of Arabidopsis thaliana, N. suaveolens, Salvia fruticosa, Salvia officinalis, and Citrus unshiu. The terpineol synthases of IV. alata and N. langsdorfii were localized in pistils and in the adaxial and abaxial epidermis of the petals. The enzyme activities reached their maxima at the second day after anthesis when flowers were fully opened and the enzyme activity in N. alata was highest at the transition from day to night （diurnal rhythm）.Nicotiana species of the section Alatae characteristically emit the floral scent compounds of the ＇cineole cassette＇ comprising 1,8-cineole, limonene, myrcene, α-pinene, β-pinene, sabinene, and α-terpineol. We successfully isolated genes of Nicotiana alata and Nicotiana langsdorfii that encoded enzymes, which produced the characteristic monoter- penes of this ＇cineole cassette＇ with α-terpineol being most abundant in the volatile spectra. The amino acid sequences of both terpineol synthases were 99% identical. The enzymes cluster in a monophyletic branch together with the closely related cineole synthase of Nicotiana suaveolens and monoterpene synthase 1 of Solanum lycopersicum. The cyclization reactions （α-terpineol to 1,8-cineole） of the terpineol synthases of N. alata and N. langsdorfii were less efficient compared to the ＇cineole cassette＇ monoterpene synthases of Arabidopsis thaliana, N. suaveolens, Salvia fruticosa, Salvia officinalis, and Citrus unshiu. The terpineol synthases of IV. alata and N. langsdorfii were localized in pistils and in the adaxial and abaxial epidermis of the petals. The enzyme activities reached their maxima at the second day after anthesis when flowers were fully opened and the enzyme activity in N. alata was highest at the transition from day to night （diurnal rhythm）.

关 键 词：Nicotiana alata Nicotiana langsdorfii cineole cassette terpineol synthase multiproduct enzyme monoterpene synthase 1 8-cineole  α-terpineol. 

分 类 号：TQ351.37[化学工程] Q592.1[生物学—生物化学]