小口径聚氨酯人工血管的制备及其力学性能、细胞相容性评价  

Assessment of the mechanical properties and biocompatibility of a new electrospun polyurethane vascular prosthesis

作　　者：贺薇[1] 胡作军[1] 徐安武[2] 殷恒讳[1] 王劲松[1] 叶洁莲[1] 王深明[1] 

机构地区：[1]中山大学附属第一医院血管外科,广东广州510080 [2]中国科学技术大学微尺度国家实验室,安徽合肥230036

出　　处：《南方医科大学学报》2011年第12期2006-2011,共6页Journal of Southern Medical University

基　　金：国家"863"项目(2007AA021904);广东省科技计划项目(2011B031300002;2010B031600055)~~

摘　　要：目的使用静电纺丝法构建一种小口径聚氨酯血管替代物,观察人工血管的微观结构,并检测其力学性能和细胞相容性。方法以聚氨酯为原料,通过静电纺丝法,以高速旋转的转轴为收集装置,制备4 mm口径的PU人工血管。探讨纺丝液质量分数对纤维直径的影响,滚轴转速对纤维排列的影响,以及质量分数、纤维取向、管壁厚度对人工血管孔隙率的影响,并检测人工血管的力学性能及细胞相容性。结果静电纺丝法制备的PU人工血管具有由多层微纳级超细纤维叠加而成的三维多孔网状结构,平均孔隙率为(51.48±4.47)%,轴向抗拉强度为(5.85±0.62)Mpa,无细胞毒性,并有利于内皮细胞黏附及增殖。结论使用静电纺丝法制备小口径聚氨酯人工血管是可行的,具有潜在的临床应用前景。Objective To develop a small-caliber vascular grafts and study its morphologies,mechanical properties and biocompatibility.Methods The effects of electrospinning conditions on the microstructure and porosity of the resulting scaffolds were investigated for preparation of a small-caliber（4 mm） polyurethane vascular grafts with optimum microstructures and mechanical properties.The mechanical properties and biocompatibility of the prepared grafts were evaluated.Results The polyurethane vascular grafts showed a three-dimensional reticular structure consisting of nanofibers,with an average porosity of（51.48±4.47）% and tensile strength of 5.85 ± 0.62 MPa.The grafts provided a better long-term support than e-PTFE graft for endothelial cell growth and endothelialization.Conclusion The polyurethane vascular prosthesis possessed favorable microstructures,excellent mechanical properties and good biocompatibility for potential clinical application.

关 键 词：人工血管 聚氨酯 电纺 力学性能 生物相容性 

分 类 号：R654.3[医药卫生—外科学]