IL28B基因多态性与慢性乙型肝炎病人对干扰素应答的关系  

Relationship between single nucleotide polymorphisms in IL28B gene and response to interferon treatment in chronic hepatitis B patients

作　　者：陈楚明[1] 周福元[1] 周元平[1] 曾艳丽[1] 李威[1] 侯金林[1] 

机构地区：[1]南方医科大学南方医院感染内科,广东广州510515

出　　处：《南方医科大学学报》2011年第12期2012-2015,共4页Journal of Southern Medical University

基　　金：国家自然科学基金(30771899)~~

摘　　要：目的探讨IL28B基因单核苷酸多态性位点(SNP)rs12979860和rs8099917与e抗原阳性慢性乙型病毒性肝炎病人对干扰素治疗应答反应性的关系。方法采集入组的82例应用干扰素治疗的病人的外周血(38例对干扰素应答,44例对干扰素不应答)。提取全基因组DNA,并扩增IL28B基因。SNP rs8099917用聚合酶链式反应-限制性片段长度多态性法分型,SNP rs12979860直接测序分型。对分型结果进行统计学分析。结果在干扰素治疗应答组,SNP rs8099917基因型TT,TG+GG的比例分别是81.6%(31/38)和18.4%(7/38),等位基因G的基因频率是9.2%(7/76),在干扰素治疗非应答组,SNP rs8099917基因型TT,TG+GG的比例分别是97.7%(43/44),2.3%(1/44),等位基因G的基因频率是1.1%(1/88),两组间的差异有统计学差异(基因型P=0.014;基因频率P=0.025)。SNP rs12979860基因型CT的比例和等位基因T频率在干扰素治疗应答组和非应答组均无显著差异(P>0.05)。结论 SNP rs8099917可能与e抗原阳性慢乙肝病人对干扰素治疗应答反应性存在相关性,等位基因G可能预示着干扰素治疗的成功应答,该位点基因型检测对于接受干扰素治疗的慢性HBV感染者可能有一定的疗效预测价值。Objective To explore the relationship between the single nucleotide polymorphisms（SNPs） of rs12979860 and rs8099917 in IL28B gene and the response to interferon treatment in hepatitis B e antigen（HBeAg）-positive patients with chronic hepatitis B patients.Methods Peripheral blood samples were collected from 82 HBeAg-positive patients with chronic hepatitis B receiving interferon treatment,including 38 with favorable response to the treatment and 44 without response.IL28B gene was amplified from the chromosomal DNA,and rs8099917 SNP was genotyped based on PCR-RLFP and rs12979860 SNP by sequencing.Results In the responsive patients,the distribution frequencies of TT and TG＋GG genotypes and allele G in SNPrs8099917 were 81.6%（31/38）,18.4%（7/38）,and 9.2%（7/76）,as compared to the frequencies of 97.7%（43/44）,2.3%（1/44）,and 1.1%（1/88） in nonresponsive patients,respectively.The frequencies showed significant differences between the responsive and nonresponsive patients（P=0.014 for genotypes and P=0.025 for allele G）.The distribution frequencies of CT genotypes and allele T in SNPrs12979860 showed no differences between the responsive and nonresponsive patients（P0.05）.Conclusion rs8099917 SNP is probably associated with the response to interferon treatment in HBeAg-positive patients with chronic hepatitis B,and Allele G may be predictive of the treatment success.

关 键 词：IL28B基因 乙型肝炎病毒 干扰素治疗 单核苷酸多态性 

分 类 号：R512.62[医药卫生—内科学]