东北梅花鹿线粒体COⅡ基因序列的遗传结构及其系统发育分析  

Genetic Structure and Phylogenetic Analysis of the mtDNA COⅡ gene in Northeastern Sika Deer

作　　者：徐佳萍[1] 荣敏[1] 杨福合[1] 邢秀梅[1] 

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学省部共建实验室特种经济动物种质资源遗传改良重点开放实验室,吉林吉林132109

出　　处：《特产研究》2011年第4期1-4,8,共5页Special Wild Economic Animal and Plant Research

基　　金：吉林省自然科学基金(20101567)

摘　　要：采用基因测序方法,对3种东北梅花鹿的COⅡ基因片段进行了PCR扩增和测序。结果表明,3种东北梅花鹿COⅡ基因片段长度均为740bp,A+T含量都大于G+C含量。3个品种单倍型多样性较高(0.673～0.717),就单个品种而言,敖东梅花鹿的遗传多样性最高。MtDNA COⅡ gene fragments of three Northeastern sika deer species were amplified using PCR method,and the products were purified and sequenced.The result showed that all the mtDNA COⅡ gene fragments had 740bp,and had greater amount of the A＋T pairs than the G＋C pairs.As a whole,the haplotype diversity was high（0.673～0.717）.In a single species,aodong sika deer had a much higher genetic diversities.

关 键 词：东北梅花鹿 mtDNACOⅡ基因 遗传结构 系统发育 

分 类 号：S865.420.3[农业科学—野生动物驯养]