HPLC法测定透骨消痛方中芍药苷和迷迭香酸的含量  

Determination of Paeoniflorin and Rosmarinic acid in Tougu Xiaotong Recipe by HPLC

作　　者：郑春松[1] 朱晓勤[1] 曾建伟[1] 谢勇[2] 李西海[1] 叶蕻芝[1] 刘献祥[1] 

机构地区：[1]福建中医药大学中西医结合研究院,福建福州350108 [2]福建中医药大学药学院,福建福州350108

出　　处：《福建中医药大学学报》2011年第6期37-40,共4页Journal of Fujian College of Traditional Chinese Medicine

基　　金：福建省科技厅重点项目资助(2009Y0029);福建省自然科学基金(2010J01196);福建省卫生厅青年科研课题(2009-1-35);福建省教育厅科技项目A类(JA09129)

摘　　要：目的建立透骨消痛方汤中芍药苷、迷迭香酸的含量测定方法。方法采用高效液相色谱法,色谱柱:Phenomenex Gemini C18(250×4.6 mm,5μm);流动相:乙腈为流动相A,0.2%的磷酸为流动相B,梯度洗脱;流速:0.8 mL/min;检测波长:芍药苷为233 nm,迷迭香酸为330 nm;柱温:35℃。结果芍药苷回归方程为Y=12.591X+53.691(r=0.9991),在12～240μg/mL范围内线性关系良好,平均加样回收率为99.59%,RSD为0.16%;迷迭香酸回归方程为Y=30.713X-212.62(r=0.9993),在6.012～60.12μg/mL范围内线性关系良好,平均加样回收率为99.01%,RSD为0.45%。结论 2种成分的峰都能有效分离,其测定方法准确、重复性良好,方中其它成分对测定无干扰。Objective To establish a HPLC method for determination of paeoniflorin and rosmarinic acid in Tougu Xiaotong recipe.Methods The separation was performed on Phenomenex Gemini C18（250×4.6 mm,5 μm） with gradient elution of acetonitrile-0.2% phosphoric acid solution as mobile phase,flow rate was 0.8 mL·min-1,the detective wavelength of paeoniflorin,rosmarinic acid was 233 nm,330 nm respectively,and the column temperature was 35℃.Results The linear ranges of paeoniflorin and rosmarinic acid were 12～240 μg·mL-1（r=0.9991）,6.012～60.12 μg·mL-1（r=0.9993） respectively,the average recoveries were 99.59%（RSD=0.16%）,99.01%（RSD=0.45%） respectively.Conclusion The peaks of the two ingredients could be segregated effectively.The method is accurate and reproducible,and there is no interference to the result from other ingredients in the recipe.

关 键 词：透骨消痛方 芍药苷 迷迭香酸 高效液相色谱 

分 类 号：R286[医药卫生—中药学]