坡缕石粘土固定辣根过氧化酶处理硝基酚废水  

The treatment of nitrophenol wastewater by horseradish peroxidase immobilized on palygorskite-based material

作　　者：舒林[1] 彭书传[1] 刘海波[1] 鲍腾[1] 刘文静[1] 

机构地区：[1]合肥工业大学资源与环境工程学院,安徽合肥230009

出　　处：《岩石矿物学杂志》2011年第6期1063-1068,共6页Acta Petrologica et Mineralogica

基　　金：教育厅安徽高校省级自然科学研究重点项目(KJ2010A060);安徽省科技成果转化基金项目(1104b0303023)

摘　　要：以坡缕石粘土、淀粉、水玻璃为原料,研究了固定化酶载体的制备工艺,探究了固定化酶载体在辣根过氧化酶(HRP)降解硝基酚废水中的作用,并探讨了反应时间、酶浓度、H2O2及硝基酚浓度对降解效果的影响。结果表明,固定化酶载体的最佳制备工艺为:水玻璃12%,淀粉20%,煅烧温度550℃,煅烧时间2 h;固定化酶对硝基酚的去除效果明显高于原酶;固定化酶去除水中硝基酚的最佳反应条件为:反应时间1.5 h,加酶量150 U,H2O2浓度0.12%,硝基酚浓度100 mg/L;固定化酶重复使用7次后对硝基酚的去除率仍高于60%。In this paper,the preparation technology of palygorskite-based compound material with palygorskite,starch,soluble glass was investigated,and the role of the compound material in the treatment of nitrophenol wastewater was studied.In addition,the effects of reaction time,addition amount of enzyme,H2O2 concentration and nitrophenol concentration on the removal of nitrophenol in wastewater by immobilized HRP was analyzed.The results showed that the optimal preparation conditions of palygorskite-based compound material were soluble glass 12%,starch 20%,calcination temperature 550℃ and calcination time 2 h.What is more important,the nitrophenol removal rate with immobilized HRP was higher than the original HRP.The optimal reaction conditions of removal of nitrophenol by immobilized HRP were reaction time 1.5 h,HRP 150 U,H2O2 concentration 0.12%,and nitrophenol concentration 100 mg/L.The nitrophenol removal rate was still above 60% when immobilized HRP was used for 7 times.

关 键 词：坡缕石粘土 硝基酚废水 固定化辣根过氧化酶 去除率 

分 类 号：P579[天文地球—矿物学] X78[天文地球—地质学]