玉米弯孢叶斑病菌ATMT转化条件优化及转GFP单孢的获得  

Optimization of Factors Affecting Agrobacterium-mediated Curvularia lunata and Characterization of Transgenic GFP Spores

作　　者：陈茂功[1,2] 林小虎[1] 李洪杰[2] 王晓鸣[2] 周印富[1] 

机构地区：[1]河北科技师范学院生命科技学院,河北秦皇岛066004 [2]中国农业科学院作物科学研究所

出　　处：《河北科技师范学院学报》2011年第3期52-57,共6页Journal of Hebei Normal University of Science & Technology

基　　金："十二.五"国家重大科技支撑计划项目"国家粮食丰产科技工程"(项目编号:2011BAD16B08)

摘　　要：为了明确玉米弯孢叶斑病菌的侵入机理,对农杆菌介导法(ATMT)转化弯孢叶斑病菌的条件进行了探究。以农杆菌AGL-1为侵染菌系、pCAMDGFP作为转化载体。结果表明,玉米弯孢叶斑病菌的ATMT最佳转化条件为:分生孢子萌发12 h后,与农杆菌在25℃黑暗条件下共培养48 h,共培养介质为微孔滤膜。转化效率可达到100～110个转化子/106个孢子,并获得转报道基因GFP的弯孢叶斑病菌单孢。In order to analyze the Curvularia lunata ingression mechanism,this study explored the main factors affecting Agrobacterium-mediated Curvularia lunata.The recombinant vector pCAMDGFP was transformed in this study into fresh conidia of Curvularia lunata via Agrobacterium tumefaciens（AGL-1）.The results showed that germination time of Curvularia lunata conidia was 12 h at 25 ℃;co-culturing time of the germinated conidia and bacteria was 48 h at 25 ℃;co-culturing media was millipore filter;and the transformation frequency was up to 100-110 transformants per 106 conidia.Transgenic GFP Curvularia lunata spores were also generated in this study.

关 键 词：农杆菌转化 玉米弯孢叶斑病菌 GFP 

分 类 号：S432[农业科学—植物病理学]