头孢菌素C高产菌株的推理选育  

Rational screening of cephalosporin C high yielding producing strains

作　　者：孟国庆[1] 赵林[1] 戴秀君[2] 李越中[3] 李世旭[1] 刘新利[1] 

机构地区：[1]山东轻工业学院山东省微生物工程重点实验室,济南250353 [2]石药集团中润制药有限公司,石家庄050041 [3]山东大学微生物技术国家重点实验室,济南250100

出　　处：《中国抗生素杂志》2011年第12期889-894,951,共7页Chinese Journal of Antibiotics

基　　金：国家科技支撑计划"抗生素战略品种的菌种选育研究"(No.2007BAI26B01);山东省自然科学基金(No.Y2008D18)

摘　　要：目的优化推理选育条件,获得头孢菌素C高产菌株。方法根据头孢菌素C的生物合成途径和代谢调控机制设计菌种推理选育方案,原始出发菌株经紫外诱变后,采用托盘琼脂柱筛选法依次对铜离子、丙二酸及头孢菌素C耐受菌株进行选育。结果最终获得一株头孢菌素C高产菌株TBC-68071,其产量较原始菌株提高了154.6%,且高产特性稳定。结论对铜离子、丙二酸及头孢菌素C耐受的顶头孢霉菌株可以高产头孢菌素C,推理选育克服了随机筛选的盲目性,提高了菌种筛选的效率。Objective To Optimize conditions of rational selection and obtain cephalosporin C high-producing strains. Methods An efficient rational selection method were designed according to the biosynthetic pathway of cephalosporin C and mechanism of metabolic regulation. After UV mutagenesis, cephalosporin C high-producing strains were screened by an agar column high-throughput method with the pressures of copper ions, malonic acid and cephalosporin C tolerance. Results The yield of cephalosporin C of the final selected strain TBC-68071 was increased 154.6% than the original strain, and the high-producinggenetic characteristics was stable. Conclusion The results showed that rational screening ofAcremonium chrysogenum overcame the blindness of random screening and improved the screening more efficiently.

关 键 词：头孢菌素C 推理选育 顶头孢霉 铜离子 丙二酸 

分 类 号：Q93-3[生物学—微生物学]