产抗生素角毛壳菌CH21的诱变育种  

Mutation breeding of antibiotics-production strain Chaetomium cupreum CH21

作　　者：王俊[1,2] 谭红[1] 钟娟[1] 周金燕[1] 舒丹[1] 杨杰[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041 [2]中国科学院研究生院,北京100039

出　　处：《中国抗生素杂志》2011年第12期895-898,911,共5页Chinese Journal of Antibiotics

基　　金：科学院重要方向项目(KSCX2-YW-N-093);科学院重要方向项目(KSCX2-EW-G-16-01);国家科技支撑计划课题"微生物代谢产物为母体的农药研究";中国科学院环境与应用微生物重点实验室(实验室代码:2010DP173074)资助课题

摘　　要：以角毛壳菌(Chaetomium cupreum)CH21的萌发孢子作为诱变材料,采用微波辐照和紫外线照射进行诱变处理,用辣椒炭疽菌(Colletotrichum capsici)作为测试菌株,结合PDA双层平板法初筛和管碟法复筛,选育高产抗生素的突变株。结果表明:最佳微波诱变条件为500W、2450MHz、15s,紫外诱变条件为15W、30cm、300s。对出发菌株CH21进行一轮微波诱变和一轮紫外诱变,筛选得到5株高产菌株,其中CH21-2-20抑菌圈直径最大,为20.20mm,较抑菌圈直径为12.50mm的出发菌株增大了62%。连续传代5次,CH21-2-20抑菌圈直径稳定在19.40～20.38mm。In order to obtain mutants with higher antibiotics yield, the germinating spores of the wild strain Chaetomium cupreum CH21 were mutated by two mutagens, microwave and UV, and screened by PDA double-plate and bioassay both using Colletotrichum capsici as indicator. The optimal mutagen dosages were determined： 500W 2450MHz under microwave irradiation for 15s, and 15W, 30cm under ultraviolet ray irradiation for 300s. CH21, the diameter of inhibiting ring of which was 12.50mm, was mutated by microwave and UV each for once. Five mutants with high yield of antibiotics were isolated. The diameter of inhibiting ring of CH21-2-20 was 20.20 mm, which was the largest and increased by 62% in comparison to CH21. Cultivated for five continuous generations, the diameter of inhibiting ring of CH21-2-20 stabilized between 19.40 mm and 20.38 mm.

关 键 词：角毛壳菌 抗生素 微波诱变 紫外诱变 

分 类 号：R978[医药卫生—药品]