一测多评法优选黄连最佳提取工艺  

Optimization of Extraction Technology of Coptis chinensis with Multi-components Quantitation by One Marker

作　　者：汪坤[1] 张振凌[1] 贾秀梅[1] 

机构地区：[1]河南中医学院,郑州450008

出　　处：《中国实验方剂学杂志》2011年第24期9-11,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：中医药行业科研专项(201007010)

摘　　要：目的:优选黄连的最佳提取工艺。方法:应用一测多评法测定黄连中表小檗碱、黄连碱、巴马汀、小檗碱的含量,通过正交试验设计,以黄连中表小檗碱、黄连碱、巴马汀及小檗碱含量之和为指标优选黄连最佳提取工艺。结果:最佳工艺条件为10倍量70%乙醇提取3次,每次1.5 h。结论:该提取工艺可以有效提取黄连中小檗碱等多种生物碱。Objective：To optimize extraction technology of Coptis chinensis.Method：Determined the content of epiberberine,coptisine,berberine and palmatine with multi-components quantitation by one marker,orthogonal design was used to optimize extraction technology of C.chinensis with total content of epiberberine,coptisine,berberine and palmatine.Result：The best extracting condition was as follows：extracted 3 times with 10 times the amount of 70% ethanol for 1.5 h each time.Conclusion： Optimum extraction technology was effective for extracting multiphasic alkaloid from C.chinensis.

关 键 词：一测多评法 黄连 提取工艺 高效液相色谱法 生物碱 

分 类 号：R283.6[医药卫生—中药学]