微波协助提取在中药饮片含量测定中的应用(3)——微波法与药典法测定土茯苓中落新妇苷含量比较  

Determination of Microwave-assisted Extraction of Chinese Cut Crude Drugs(3) Comparison of Determination of Astilbin from Smilax glabra between Microwave-assisted Extraction and Chinese Pharmacopeia Method

作　　者：方婧[1] 付梅红[1] 杨洪军[1] 张贝贝[1] 王祝举[1] 杨岚[1] 杨雪燕[2] 张梦阳[2] 张康丁 张东[1] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]沈阳药科大学,沈阳110016 [3]邢台医学高等专科学校,河北邢台054000

出　　处：《中国实验方剂学杂志》2011年第24期43-45,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金："重大新药创制"科技重大专项(2009ZX09308-003);国家科技支撑计划子课题(2006BAI09B07-02)

摘　　要：目的:建立微波协助提取法提取土茯苓中有效成分落新妇苷含量测定方法,比较微波协助提取法与药典法提取落新妇苷优势。方法:采用Dikma Diamonsil C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.1%冰醋酸水溶液(39∶61),检测波长291 nm,柱温30℃,流速1 mL.min-1。结果:微波提取时间10 min,提取温度60℃,提取溶剂60%乙醇。落新妇苷在0.04～0.8μg呈良好线性关系(r=0.999 9)。平均回收率99.80%(n=6)。结论:微波提取法较药典法提取落新妇苷更简便快速、结果准确,为一种符合环保绿色理念的含量测定方法,可用于检测土茯苓饮片中有效成分落新妇苷含量。Objective：To establish a microwave-assisted extraction method for the extraction of the astilbin from Smilax glabra.Comparison of the determination of astilbin from Smilax glabra between microwave-assisted extraction and Chinese pharmacopeia method.Method：Dikma Diamonsil C18 column（4.6 mm×150 mm,5μm）was used.The mobile phase of methanol-0.1%acetic acid-water（39∶61）.The temperature of column was 30 ℃.The flow rate was 1.0 mL·min-1 and the detection wavelength was at 291 nm.Result：Microwave extractive time was 10 min,the temperature was 60 ℃,the extraction solvent was 60%ethanol.The calibration curve was in good linearity with in the range from 0.04 to 0.8 μg（r=0.999 9）.The average recovery was 99.80%（n=6）.Conclusion：The method is simple,rapid,as well as precise and reliable,and can be used for the determination of astilbin from Smilax glabra.

关 键 词：微波提取 土茯苓 落新妇苷 含量测定 

分 类 号：R284.1[医药卫生—中药学]