不同产地生制狗脊中多糖的比较分析  

Determination of Polysaccharides in Cibotium Barometz and Its Processed Product from Various Cultivation Locations

作　　者：鞠成国[1,2] 章琦[1] 于海涛[1] 高倩[1] 史俊[1] 贾天柱[1,2] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2011年第24期46-48,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30973938)

摘　　要：目的:建立狗脊中多糖的含量测定方法,比较不同产地生制狗脊多糖的含量。方法:采用苯酚-硫酸法测定狗脊中多糖的含量,利用单糖和多糖的换算因子计算多糖含量,测定波长为489 nm。结果:在2.46～14.79 mg.L-1葡萄糖浓度C与吸光度A线性关系良好(r=0.999 5),平均回收率为98.7%,RSD 1.48%。不同产地的生狗脊多糖含量在2.48%～10.06%,制狗脊多糖含量在5.56%～15.17%。结论:该法简单、方便、准确、可靠,可以用来测定狗脊中多糖含量。不同产地狗脊中多糖的含量差异较大,其中福建产狗脊中多糖含量最高,狗脊经炮制后多糖的含量升高。Objective： To establish a method for determining polysaccharides in Cibotium barometz,then compare different place of production the content of polysaccharides in Cibotium barometz and its processed product.Method： The content of the polysaccharides in Cibotium barometz was determined by phenol-sulfuric acid method,and calculated using a conversion factor,and the determination of the wavelength was 489 nm.Result： The glucose concentration value C and absorbance A were linear in the range of 2.46-14.79 mg·L-1（r=0.999 5）,The average recovery was 98.7%,RSD 1.48%.The content of polysaccharides in Cibotium barometz from various cultivation locations varied from 2.48%-10.06%,and 5.56%-15.17% in its processed product.Conclusion： The method is simple,effective and feasible.It can be used for the determination of the content of polysaccharides in Cibotium barometz.The content of polysaccharides in Cibotium barometz which were collected at Fujian City was the highest.There is great diference in polysaccharides content in Cibotium barometz from various cultivation locations,and the content of polysaccharides was increased after processed.

关 键 词：狗脊 炮制 多糖 含量测定 

分 类 号：R284.1[医药卫生—中药学]