胆木叶提取部位群的抗炎镇痛作用  

Anti-inflammatory and Analgesic Effects of Extracts from the Leaves of Nauclea officinalis

作　　者：曹亮[1,2,3] 李娜[1,2,3] 姜雅琼[2,3] 丁岗[1,2,3] 王振中[2,3] 萧伟[2,3] 

机构地区：[1]江苏中康药物科技有限公司,南京210017 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国实验方剂学杂志》2011年第24期124-127,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家科技重大专项-重大新药创制项目(2009ZX09103-379);江苏省科技支撑计划项目(SBE200970060)

摘　　要：目的:观察胆木叶提取部位群(ELN)的镇痛和抗炎作用。方法:取ICR小鼠随机分为正常组、模型组、阳性药物[三七伤药(0.39 g.kg-1)/阿司匹林(0.26 g.kg-1)]组、胆木浸膏片1.56 g.kg-1组、ELN(0.390,0.195,0.098 g.kg-1)组。每天1次,连续ig给药3 d。末次给药后1 h选用热板法、扭体法、二甲苯致耳肿胀、醋酸致腹腔炎症方法观察镇痛及抗炎作用;取SD大鼠随机分为正常组、模型组、胆木浸膏片(0.78 g.kg-1)组、ELN(0.195,0.098,0.049 g.kg-1)组。每天1次,连续ig给药3 d。末次给药后1 h选用角叉菜胶诱导足肿胀方法观察抗炎作用。结果:ELN(主要指标性成分异长春花苷内酰胺含量16%左右)能显著延长小鼠的疼痛反应时间,明显减少小鼠的扭体次数;抑制醋酸致小鼠腹腔毛细血管通透性增高,减轻小鼠耳肿胀和大鼠足跖肿胀反应;能明显减少大鼠角叉菜胶性炎症渗出液中前列腺素E2(PGE2)含量。结论:ELN具有显著的镇痛和抗炎作用,其机制可能与抑制PGE2产生与释放有关。Objective： To study the anti-inflammatory and analgesic effects of extracts from the leaves of Nauclea officinalis Pierre ex Pitard（ELN）.Method： ICR mice were divided into 7 groups randomly,namely normal group,model group（0.39 g·kg^-1）,Sanqi Shangyao pian group（1.56 g·kg^-1）,aspirin group,Danmu Jingao pian group and ELN（0.390,0.195,0.098 g·kg^-1）groups.Each drug was administrated to mice orally once a day for 3 days.1 h after the last administration,analgesic and anti-inflammatory effects were observed by methods of hot-plate,acetic-acid induced writhing,xylene-induced ear edema and acetic acid-induced peritoneal capillary permeability,respectively.The model of carrageenin-induced paws edema in rats were also selected to observe the anti-inflamatory activity 1 h after the last administration of ELN（0.195,0.098,0.049 g·kg^-1）.Contol group,model group and Danmu Jingao pian group（0.78 g·kg^-1） were parallelly setted.Drugs was administrated to rats orally once a day for 3 days before determination.Result： ELN（the content of its main constituent strictosamide is about 16%） could prolong the latent period of pain and decrease the number of writhing induced by acetic acid markedly.The permeability increase of blood capillary induced by acetic acid in mice,the swelling of ear induced by xylene in mice,as well as the edema of paw induced by carrageenin in rats were also significantly inhibited by ELN respectively.Additionally,the amounts of prostaglandin E2（PGE2） in the inflammatory diffusate induced by carrageenin were significantly reduced.Conclusion： ELN has significant analgesic and anti-inflammatory activities which may correlate with the inhibition of production and relase of PGE2.

关 键 词：胆木叶 抗炎 镇痛 前列腺素E2 

分 类 号：R285.5[医药卫生—中药学]